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A plant cell wall-associated kinase encoding gene is dramatically downregulated during nematode infection of potato.

Authors:
Shiyan Chen Lili Cui Xiaohong Wang

Plant Signal Behav 2022 12 29;17(1):2004026. Epub 2021 Dec 29.

School of Integrative Plant Science, Cornell University, Ithaca, NY, USA.

Plant cell wall associated kinases (WAKs) and WAK-like kinases (WAKLs) have been increasingly recognized as important regulators of plant immunity against various plant pathogens. However, the role of the / family in plant-nematode interactions remains to be determined. Here, we analyzed a -encoding gene () from potato (). The encoded protein contains domains characteristic of WAK/WAKL proteins and shows the highest similarity to SlWAKL2 from tomato (). We thus named the gene as . Phylogenetic analysis of a wide range of plant WAKs/WAKLs further revealed close similarity of StWAKL2 to three WAK/WAKL proteins demonstrated to play a role in disease resistance. To gain insights into the potential regulation and function of , transgenic potato lines containing the promoter fused to the β-glucuronidase (GUS) reporter gene were generated and used to investigate expression during plant development and upon nematode infection. Histochemical analyses revealed that has specific expression patterns in potato leaf and root tissues. During nematode infection, GUS activity was mostly undetected at nematode infection sites over the course of nematode parasitism, although strong GUS activity was observed in root tissues adjacent to the infection region. Furthermore, mining of the transcriptomic data derived from cyst nematode infection of Arabidopsis roots identified a few / genes, including a homologue, found to be significantly down-regulated in nematode-induced feeding sites. These results indicated that specific suppression of / genes in nematode-induced feeding sites might be crucial for cyst nematodes to achieve successful infection of host plants. Further studies are needed to uncover the role of / genes in plant defenses against nematode infection.

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http://dx.doi.org/10.1080/15592324.2021.2004026DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8928814PMC
December 2022

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