Pubfacts - Scientific Publication Data
  • Categories
  • |
  • Journals
  • |
  • Authors
  • Login
  • Categories
  • Journals

Search Our Scientific Publications & Authors

Publications
  • Publications
  • Authors
find publications by category +
Translate page:

Magnetic resonance reveals mitochondrial dysfunction and muscle remodelling in spinal muscular atrophy.

Authors:
Laura E Habets Bart Bartels Fay-Lynn Asselman Melissa T Hooijmans Sandra van den Berg Aart J Nederveen W Ludo van der Pol Jeroen A L Jeneson

Brain 2022 05;145(4):1422-1435

Centre for Child Development, Exercise and Physical Literacy, Wilhelmina Children's Hospital, University Medical Centre Utrecht, P.O. Box 85090, 3508 AB Utrecht, the Netherlands.

Genetic therapy has changed the prognosis of hereditary proximal spinal muscular atrophy, although treatment efficacy has been variable. There is a clear need for deeper understanding of underlying causes of muscle weakness and exercise intolerance in patients with this disease to further optimize treatment strategies. Animal models suggest that in addition to motor neuron and associated musculature degeneration, intrinsic abnormalities of muscle itself including mitochondrial dysfunction contribute to the disease aetiology. To test this hypothesis in patients, we conducted the first in vivo clinical investigation of muscle bioenergetics. We recruited 15 patients and 15 healthy age and gender-matched control subjects in this cross-sectional clinico-radiological study. MRI and 31P magnetic resonance spectroscopy, the modality of choice to interrogate muscle energetics and phenotypic fibre-type makeup, was performed of the proximal arm musculature in combination with fatiguing arm-cycling exercise and blood lactate testing. We derived bioenergetic parameter estimates including: blood lactate, intramuscular pH and inorganic phosphate accumulation during exercise, and muscle dynamic recovery constants. A linear correlation was used to test for associations between muscle morphological and bioenergetic parameters and clinico-functional measures of muscle weakness. MRI showed significant atrophy of triceps but not biceps muscles in patients. Maximal voluntary contraction force normalized to muscle cross-sectional area for both arm muscles was 1.4-fold lower in patients than in controls, indicating altered intrinsic muscle properties other than atrophy contributed to muscle weakness in this cohort. In vivo31P magnetic resonance spectroscopy identified white-to-red remodelling of residual proximal arm musculature in patients on the basis of altered intramuscular inorganic phosphate accumulation during arm-cycling in red versus white and intermediate myofibres. Blood lactate rise during arm-cycling was blunted in patients and correlated with muscle weakness and phenotypic muscle makeup. Post-exercise metabolic recovery was slower in residual intramuscular white myofibres in patients demonstrating mitochondrial ATP synthetic dysfunction in this particular fibre type. This study provides the first in vivo evidence in patients that degeneration of motor neurons and associated musculature causing atrophy and muscle weakness in 5q spinal muscular atrophy type 3 and 4 is aggravated by disproportionate depletion of myofibres that contract fastest and strongest. Our finding of decreased mitochondrial ATP synthetic function selectively in residual white myofibres provides both a possible clue to understanding the apparent vulnerability of this particular fibre type in 5q spinal muscular atrophy types 3 and 4 as well as a new biomarker and target for therapy.

Download full-text PDF

Source
http://dx.doi.org/10.1093/brain/awab411DOI Listing
May 2022

Publication Analysis

Top Keywords

muscle weakness
20
muscular atrophy
16
spinal muscular
16
muscle
14
blood lactate
12
magnetic resonance
12
patients
9
atp synthetic
8
fibre type
8
proximal arm
8
arm musculature
8
mitochondrial atp
8
associated musculature
8
intramuscular inorganic
8
inorganic phosphate
8
resonance spectroscopy
8
white myofibres
8
phosphate accumulation
8
mitochondrial dysfunction
8
atrophy
7

Keyword Occurance

Similar Publications

Systemic considerations for the surgical treatment of spinal metastatic disease: a scoping literature review.

Authors:
Mark A MacLean Charles J Touchette Miltiadis Georgiopoulos Tristan Brunette-Clément Fahad H Abduljabbar Christopher P Ames Chetan Bettegowda Raphaele Charest-Morin Nicolas Dea Michael G Fehlings Ziya L Gokaslan C Rory Goodwin Ilya Laufer Cordula Netzer Laurence D Rhines Arjun Sahgal John H Shin Daniel M Sciubba Byron F Stephens Daryl R Fourney Michael H Weber

Lancet Oncol 2022 Jul;23(7):e321-e333

Spine Surgery Program, Department of Surgery, Montreal General Hospital, McGill University Health Center, Montreal, QC, Canada. Electronic address:

Systemic assessment is a pillar in the neurological, oncological, mechanical, and systemic (NOMS) decision-making framework for the treatment of patients with spinal metastatic disease. Despite this importance, emerging evidence relating systemic considerations to clinical outcomes following surgery for spinal metastatic disease has not been comprehensively summarised. We aimed to conduct a scoping literature review of this broad topic. Read More

View Article and Full-Text PDF
July 2022
Similar Publications

Validity and reproducibility of C-Mill walking-adaptability assessment in polio survivors.

Authors:
Jana Tuijtelaars Merel-Anne Brehm Frans Nollet Melvyn Roerdink

Gait Posture 2022 Jun 15;96:314-321. Epub 2022 Jun 15.

Department of Human Movement Sciences, Faculty of Behavioural and Human Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, Van der Boechorststraat 7, Amsterdam, the Netherlands.

Background: The C-Mill interactive treadmill allows for a safe walking-adaptability assessment, unveiling reduced walking adaptability in polio survivors compared to healthy individuals, possibly related to their high fall rate. However, evidence on its validity and reproducibility is scarce.

Research Question: What is the validity and reproducibility of C-Mill walking-adaptability assessment in polio survivors?

Methods: Polio survivors with a history and/or fear of falling (n = 46) performed two walking-adaptability assessments, 1-2 weeks apart, including target-stepping tests (with 0%, 20% and 30% inter-target variance) and obstacle-avoidance tests (anticipatory and reactive). Read More

View Article and Full-Text PDF
June 2022
Similar Publications

Generation of an induced pluripotent stem cell line (ZZUi036-A) derived from skin fibroblasts of a Neuronal intranuclear inclusion disease patient with GGC repeat expansion in the NOTCH2NLC gene.

Authors:
Jiadi Li Yu Fan Liyuan Fan Fen Liu Xiaoyan Hao Mengjie Li Chengyuan Mao Yuming Xu Changhe Shi

Stem Cell Res 2022 Jun 15;63:102844. Epub 2022 Jun 15.

Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address:

Neuronal intranuclear inclusion disease (NIID) is a neurodegenerative disease characterized by cognitive impairment, extrapyramidal symptoms, white matter lesions and muscle weakness. The cause of NIID is a repeat amplification of a GGC mutation in the 5 ' untranslated region (UTR) of the NOTCH2NLC gene. Using the non-integrating Sendai virus to deliver the Klf4, OCT3/4, SOX2 and C-MYC factors, fibroblasts obtained from a NIID patient were reprogrammed to generate an induced pluripotent stem cell (iPSC) line (ZZUi036-A). Read More

View Article and Full-Text PDF
June 2022
Similar Publications

Early presentation of lower urinary tract and bowel dysfunction in sporadic amyotrophic lateral sclerosis: A case report.

Authors:
Masataka Nakamura Kentaro Nakayama Aya Murakami Satoshi Morise Satoshi Kaneko Hirofumi Kusaka Yusuke Yakushiji

eNeurologicalSci 2022 Sep 18;28:100413. Epub 2022 Jun 18.

Department of Neurology, Kansai Medical University, 2-5-1 Shinmachi, Hirakata, Osaka 573-1010, Japan.

An autopsy case of sporadic amyotrophic lateral sclerosis (ALS) with lower urinary tract (LUT) and bowel dysfunction is reported. The dysfunction occurred simultaneously with motor neuron symptoms in the early stages of the illness. A 75-year-old man developed exertional dyspnea and constipation following weight loss. Read More

View Article and Full-Text PDF
September 2022
Similar Publications

[Spinal arteriovenous malformations].

Authors:
A Udelhoven M Kettner W Reith

Radiologie (Heidelb) 2022 Jun 29. Epub 2022 Jun 29.

Klinik für Diagnostische und Interventionelle Neuroradiologie, Universitätsklinikum des Saarlandes (Homburg), Neurozentrum (Geb. 90), Kirrbergerstr., 66421, Homburg/Saar, Deutschland.

Spinal vascular malformations include various entities such as spinal arteriovenous malformations (AVM) and represent a rare disease. Patients may present with acute or progressive sensorimotor deficits and/or nonspecific symptoms such as hypoesthesia or paresthesia, diffuse back and muscle pain, or feelings of weakness. When spinal arteriovenous malformation is suspected, magnetic resonance imaging (MRI) is the first diagnostic step to rule out other differential diagnoses, followed by a selective spinal angiography. Read More

View Article and Full-Text PDF
June 2022
Similar Publications
}
© 2022 PubFacts.
  • About PubFacts
  • Privacy Policy
  • Sitemap