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Metab Eng 2021 Apr 19. Epub 2021 Apr 19.

Biochemical Engineering Institute, Saarland University, Campus A1.5, D-66123 Saarbrücken (Germany). Electronic address:

We describe a systematic approach to establish predictive models of CHO cell growth, cell metabolism and monoclonal antibody (mAb) formation during biopharmaceutical production. The prediction is based on a combination of an empirical metabolic model connecting extracellular metabolic fluxes with cellular growth and product formation with mixed Monod-inhibition type kinetics that we generalized to every possible external metabolite. We describe the maximum specific growth rate as a function of the integral viable cell density (IVCD). Read More

Plant Physiol 2019 Jul;180(4):1912-1929

Departament of Botany, University of Brasília, Campus Darcy Ribeiro, Asa Norte, Brasília, Brazil, 70910-900.

Until they become photoautotrophic juvenile plants, seedlings depend upon the reserves stored in seed tissues. These reserves must be mobilized and metabolized, and their breakdown products must be distributed to the different organs of the growing seedling. Here, we investigated the mobilization of soybean (Glycine max) seed reserves during seedling growth by initially constructing a genome-scale stoichiometric model for this important crop plant and then adapting the model to reflect metabolism in the cotyledons and hypocotyl/root axis (HRA). Read More

Cancer Nurs 2021 Apr 21. Epub 2021 Apr 21.

Author Affiliations: Nursing School of Jilin University (Ms Fu, Dr Li Ms Wei, Ms Wang, Ms Dong, and Dr Peng); and Department of Breast Oncology, Jilin Provincial Cancer Hospital, Changchun, the People's Republic of China (Dr Zhou).

Background: Some empirical investigations try to describe the factors that affect the physical activity of breast cancer survivors, but these investigations rarely focus on the basic psychological needs or motivation regulation of breast cancer survivors in physical activity.

Objective: The aim of this study was to construct structural equation modeling of the self-determination theory and upper limb factors to predict the physical activity of breast cancer patients during chemotherapy.

Methods: A total of 236 breast cancer survivors completed measures of basic psychological needs, behavioral regulation in exercise, symptoms and dysfunction of upper limb factors, and physical activity. Read More

Sci Rep 2021 Apr 20;11(1):8544. Epub 2021 Apr 20.

Bioinformatics, Institute for Biochemistry and Biology, University of Potsdam, 14476, Potsdam, Germany.

Thermodynamic metabolic flux analysis (TMFA) can narrow down the space of steady-state flux distributions, but requires knowledge of the standard Gibbs free energy for the modelled reactions. The latter are often not available due to unknown Gibbs free energy change of formation [Formula: see text], of metabolites. To optimize the usage of data on thermodynamics in constraining a model, reaction lumping has been proposed to eliminate metabolites with unknown [Formula: see text]. Read More

Clin Pharmacol Drug Dev 2021 Apr 19. Epub 2021 Apr 19.

Amicus Therapeutics, Inc., Cranbury, New Jersey, USA.

Approved therapies for Fabry disease (FD) include migalastat, an oral pharmacological chaperone, and agalsidase beta and agalsidase alfa, 2 forms of enzyme replacement therapy. Broad tissue distribution may be beneficial for clinical efficacy in FD, which has severe manifestations in multiple organs. Here, migalastat and agalsidase beta biodistribution were assessed in mice and modeled using physiologically based pharmacokinetic (PBPK) analysis, and migalastat biodistribution was subsequently extrapolated to humans. Read More