Pubfacts - Scientific Publication Data
  • Categories
  • |
  • Journals
  • |
  • Authors
  • Login
  • Categories
  • Journals

Search Our Scientific Publications & Authors

Publications
  • Publications
  • Authors
find publications by category +
Translate page:

A unified alternative telomere-lengthening pathway in yeast survivor cells.

Authors:
Zachary W Kockler Josep M Comeron Anna Malkova

Mol Cell 2021 Feb 19. Epub 2021 Feb 19.

Department of Biology, University of Iowa, Iowa City, IA 52245, USA; Interdisciplinary Graduate Program in Genetics, University of Iowa, Iowa City, IA 52245, USA. Electronic address:

Alternative lengthening of telomeres (ALT) is a recombination process that maintains telomeres in the absence of telomerase and helps cancer cells to survive. Yeast has been used as a robust model of ALT; however, the inability to determine the frequency and structure of ALT survivors hinders understanding of the ALT mechanism. Here, using population and molecular genetics approaches, we overcome these problems and demonstrate that contrary to the current view, both RAD51-dependent and RAD51-independent mechanisms are required for a unified ALT survivor pathway. This conclusion is based on the calculation of ALT frequencies, as well as on ultra-long sequencing of ALT products that revealed hybrid sequences containing features attributed to both recombination pathways. Sequencing of ALT intermediates demonstrates that recombination begins with Rad51-mediated strand invasion to form DNA substrates that are matured by a Rad51-independent ssDNA annealing pathway. A similar unified ALT pathway may operate in other organisms, including humans.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.molcel.2021.02.004DOI Listing
February 2021

Publication Analysis

Top Keywords

alt
9
unified alt
8
sequencing alt
8
problems demonstrate
4
rad51-mediated strand
4
approaches overcome
4
overcome problems
4
recombination rad51-mediated
4
view rad51-dependent
4
rad51-dependent rad51-independent
4
current view
4
contrary current
4
strand invasion
4
demonstrate contrary
4
genetics approaches
4
dna substrates
4
understanding alt
4
survivors hinders
4
alt survivors
4
substrates matured
4

Keyword Occurance

Similar Publications

Dietary astaxanthin augments disease resistance of Asian seabass, Lates calcarifer (Bloch, 1790) against Vibrio alginolyticus infection.

Authors:
Keng Chin Lim Fatimah Md Yusoff Mohamed Shariff Mohd Salleh Kamarudin

Fish Shellfish Immunol 2021 Apr 7. Epub 2021 Apr 7.

Department of Aquaculture, Faculty of Agriculture, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia; International Institute of Aquaculture and Aquatic Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia.

This investigation describes the impacts of dietary provisioning with astaxanthin on hemato-biochemistry, non-specific immunity, and disease resistance of the Asian seabass, Lates calcarifer against the virulent Vibrio alginolyticus, with specific reference to dose-response associations and variations over different post-infection periods (0-, 7-, and 14-day). Triplicate groups of fish weighing 28 g, on average, were fed various diets (C, the control or astaxanthin-free; AXT50, 50 mg astaxanthin kg diet; AXT100, 100 mg astaxanthin kg diet; and AXT150, 150 mg astaxanthin kg diet) for 90 days and subsequently challenged with V. alginolyticus at the end of the feeding period. Read More

View Article and Full-Text PDF
April 2021
Similar Publications

Increased toxicity and retention of Perflourooctane Sulfonate (PFOS) in humanized CYP2B6-Transgenic mice compared to Cyp2b-null mice is relieved by a High-Fat Diet (HFD).

Authors:
Matthew C Hamilton Melissa M Heintz Marisa Pfohl Emily Marques Lucie Ford Angela L Slitt William S Baldwin

Food Chem Toxicol 2021 Apr 7:112175. Epub 2021 Apr 7.

Environmental Toxicology Program, Clemson University, Clemson, SC, 29634. Electronic address:

PFOS is a persistent, fluorosurfactant used in multiple products. Murine Cyp2b's are induced by PFOS and high-fat diets (HFD) and therefore we hypothesized that human CYP2B6 may alleviate PFOS-induced steatosis. Cyp2b-null and hCYP2B6-Tg mice were treated with 0, 1, or 10mg/kg/day PFOS by oral gavage for 21-days while provided a chow diet (ND) or HFD. Read More

View Article and Full-Text PDF
April 2021
Similar Publications

Fine-scale population structure in five rural populations from the Spanish Eastern Pyrenees using high-coverage whole-genome sequence data.

Authors:
Iago Maceda Miguel Martín Álvarez Georgios Athanasiadis Raúl Tonda Jordi Camps Sergi Beltran Agustí Camps Jordi Fàbrega Josefina Felisart Joan Grané José Luis Remón Jordi Serra Pedro Moral Oscar Lao

Eur J Hum Genet 2021 Apr 9. Epub 2021 Apr 9.

CNAG-CRG, Centre for Genomic Regulation, C/ Baldiri i Reixach 4, 08028, Barcelona, Spain.

The area of the Spanish Pyrenees is particularly interesting for studying the demographic dynamics of European rural areas given its orography, the main traditional rural condition of its population and the reported higher patterns of consanguinity of the region. Previous genetic studies suggest a gradient of genetic continuity of the area in the West to East axis. However, it has been shown that micro-population substructure can be detected when considering high-quality NGS data and using spatial explicit methods. Read More

View Article and Full-Text PDF
April 2021
Similar Publications

Normalization of blood clotting characteristics using prothrombin complex concentrate, fibrinogen and FXIII in an albumin based fluid: experimental studies in thromboelastometry.

Authors:
Tobias Koller Nadia Kinast Andres Guilarte Castellanos Sergio Perez Garcia Pilar Paniagua Iglesias Xavi León Vintro Jose Mateo Arranz Noelia Vilalta Seto Ma Victòria Moral García Ana Belén Moreno-Castaño Jose Aznar-Salatti Gines Escolar Albaladejo Maribel Diaz-Ricart

Scand J Trauma Resusc Emerg Med 2021 Apr 9;29(1):57. Epub 2021 Apr 9.

Hematopathology, Pathology Department, CDB, Hospital Clinic, IDIBAPS, University of Barcelona, Barcelona, Spain.

Background: Colloid fluids supplemented with adequate combinations of coagulation factor concentrates with the capability to restore coagulation could be a desirable future treatment component in massive transfusion.

Methods: Starting from a coagulation factor and blood cell-free albumin solution we added Prothrombin Complex Concentrate, Fibrinogen Concentrate and Factor XIII in different combinations and concentrations to analyze their properties to restore thromboelastometry parameters without the use of plasma. Further analysis under the presence of platelets was performed for comparability to whole blood conditions. Read More

View Article and Full-Text PDF
April 2021
Similar Publications

Dioscin ameliorates methotrexate-induced liver and kidney damages via adjusting miRNA-145-5p-mediated oxidative stress.

Authors:
Y Li M Gao L-H Yin L-N Xu Y Qi Pengyuan Sun J-Y Peng

Free Radic Biol Med 2021 Apr 6. Epub 2021 Apr 6.

College of Pharmacy, Dalian Medical University, Western 9 Lvshunnan Road, Dalian, 116044, China; Key Laboratory for Basic and Applied Research on Pharmacodynamic Substances of Traditional Chinese Medicine of Liaoning Province, Dalian Medical University, Dalian, China. Electronic address:

Dioscin, one natural product, has various pharmacological actions. However, its effects on methotrexate (MTX)-induced hepatorenal damages still remain unknown. In the present study, the data manifested that dioscin restored the viabilities of L-02 and NRK-52E cells, reduced ALT, AST, Cr, BUN levels, and ameliorated histopathological changes of liver and kidney. Read More

View Article and Full-Text PDF
April 2021
Similar Publications
© 2021 PubFacts.
  • About PubFacts
  • Privacy Policy
  • Sitemap