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Development of Monoclonal Antibodies against Pea Globulins for Multiplex Assays Targeting Legume Proteins.

Authors:
Norbert Lidzba Verónica García Arteaga Andreas Schiermeyer Heide Havenith Isabel Muranyi Stefan Schillberg Jörg Lehmann Elke Ueberham

J Agric Food Chem 2021 Mar 25;69(9):2864-2874. Epub 2021 Feb 25.

Fraunhofer Institute for Cell Therapy and Immunology IZI, Perlickstraße 1, Leipzig 04103, Germany.

Legume proteins are widely used as food ingredients, but only some (soybean, lupin, and peanut) must be declared under consumer safety regulations to protect allergy sufferers. It is not yet mandatory to declare pea proteins as allergens even though they are predicted to be allergenic based on cross-reactivity in sensitized people. The processing of legume proteins can modify their allergenic properties and hence the need for specific and precise methods for the detection of all major legume allergens. There are many commercially available tests for known food allergens but not for ingredients that are yet to be classified as allergenic. We therefore generated sets of pea-specific antibodies targeting globulins to be used in a multiplex assay for the simultaneous detection of soybean, lupin, peanut, and pea proteins. We focused on the 7S globulin family, which is the least conserved among the four legumes, allowing the specific detection of proteins from each species. Having confirmed the specificity and sensitivity of the multiplex assay, we evaluated different processing steps for proteins rich in pea globulins to demonstrate the impact of food processing on antibody binding. Our sensitive multiplex assay provides a fast and reliable method for the specific detection of soybean, lupin, peanut, and pea allergens and is therefore ideal for food safety and authenticity testing applications.

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http://dx.doi.org/10.1021/acs.jafc.0c07177DOI Listing
March 2021

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