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IEEE Trans Ultrason Ferroelectr Freq Control 2021 Apr 12;PP. Epub 2021 Apr 12.

There is significant acoustic impedance contrast between the cortical bone and surrounding soft tissue, resulting in difficulty for ultrasound penetration into bone tissue with high frequency. It is challenging for the conventional pulse-echo modalities to give accurate cortical bone images using uniform sound velocity model. To overcome these limitations, an ultrasound imaging method called full-matrix Fourier-domain synthetic aperture based on velocity inversion (FM-FDSA-VI) was developed to provide accurate cortical bone images. Read More

IEEE Trans Med Imaging 2021 Apr 12;PP. Epub 2021 Apr 12.

Data truncation is a common problem in computed tomography (CT). Truncation causes cupping artifacts inside the field-of-view (FOV) and anatomical structures missing outside the FOV. Deep learning has achieved impressive results in CT reconstruction from limited data. Read More

Med Phys 2021 Apr 12. Epub 2021 Apr 12.

Hospital Universitario Virgen de la Arrixaca, ctra. Madrid-Cartagena, 30120, El Palmar, Spain.

Purpose: Using linear transformations of the acquired data can expand the study of detectability in an imaging system. From one image, an appropriate transformation will produce a set of signals with different contrast and different frequency contents. In this work this strategy is explored to present a task-based test for the detectability of an x-ray imaging system. Read More

J Vis Exp 2021 Mar 26(169). Epub 2021 Mar 26.

College of Optometry, University of Houston; Children's Nutrition Center, Baylor College of Medicine.

Serial block-face scanning electron microscopy (SBF-SEM) allows for the collection of hundreds to thousands of serially-registered ultrastructural images, offering an unprecedented three-dimensional view of tissue microanatomy. While SBF-SEM has seen an exponential increase in use in recent years, technical aspects such as proper tissue preparation and imaging parameters are paramount for the success of this imaging modality. This imaging system benefits from the automated nature of the device, allowing one to leave the microscope unattended during the imaging process, with the automated collection of hundreds of images possible in a single day. Read More

J Vis Exp 2021 Mar 29(169). Epub 2021 Mar 29.

Departments of Research Pathology, Genentech;

Tumor enrichment in low tumor content tissues, those below 20% tumor content depending on the method, is required to generate quality data reproducibly with many downstream assays such as next generation sequencing. Automated tissue dissection is a new methodology that automates and improves tumor enrichment in these common, low tumor content tissues by decreasing the user-dependent imprecision of traditional macro-dissection and time, cost, and expertise limitations of laser capture microdissection by using digital image annotation overlay onto unstained slides. Here, digital hematoxylin and eosin (H&E) annotations are used to target small tumor areas using a blade that is 250 µm in diameter in unstained formalin fixed paraffin embedded (FFPE) or fresh frozen sections up to 20 µm in thickness for automated tumor enrichment prior to nucleic acid extraction and whole exome sequencing (WES). Read More