Search Our Scientific Publications & Authors

Publications
find publications by category +
Translate page:

[Effects of hnRNP E1 on expression of early genes E2, E6 of HPV16 and biological function in cervical cancer cells].

Authors:
L Song L Ding M J Feng X X Li W Gao Z Qi H Liu M Wang J T Wang

Zhonghua Liu Xing Bing Xue Za Zhi 2021 Feb;42(2):321-326

Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, China.

To explore the effects of hnRNP E1 on the expression of early genes E2, E6 of HPV16 and the biological function in cervical cancer SiHa cell lines. The cell experiments were carried out in cervical cancer cell lines SiHa. The expression levels of E2, E6 mRNA and protein of HPV16 were detected by Real-time PCR and Western blot, respectively, before and after up-regulating hnRNP E1. Meanwhile, the cell proliferation, cycle and apoptosis were evaluated by CCK-8 and flow cytometry. Data analyses were performed using SPSS 22.0 and Graphpad Prism 7.0 software. Compared with the blank and the blank plasmid group, the cells activity and proliferation decreased at 24, 48 and 72 h after up-regulating hnRNP E1 (<0.05), while the percentage of cells in G0/G1 phase increased and the percentage in S and G2/M phase and proliferation index decreased (<0.05). Moreover, the late apoptotic rate and the total apoptotic rate increased (<0.05). The expression levels of E6 mRNA and protein of HPV16 in hnRNP E1 up-regulated group were significantly lower than that in both blank group and blank plasmid group, the differences were significant (<0.05), showing the tendency of cells proliferation index decrease and total apoptotic rate increase with decreased HPV16 E6 expression. There were no significant differences in the expression of E2 mRNA of HPV16 among the three groups (=0.427), and no E2 protein of HPV16 was detected. hnRNP E1 could inhibit the transcription and translation of E6 oncogene of HPV16 and further inhibit the proliferation and promote apoptosis of cervical cancer cells, suggesting that hnRNP E1 might be a potential target marker to prevent cervical lesions. But no association between hnRNP E1 and HPV16 E2 was found in SiHa cells.

Download full-text PDF

 Source
http://dx.doi.org/10.3760/cma.j.cn112338-20191009-00723DOI Listing
February 2021

Publication Analysis

Top Keywords

cervical cancer
12
biological function
8
genes hpv16
8
function cervical
8
cell lines
8
up-regulating hnrnp
8
early genes
8
hpv16 biological
8
expression early
8
hnrnp expression
8
blank plasmid
4
detected real-time
4
real-time pcr
4
pcr western
4
expression levels
4
220 graphpad
4
group cells
4
levels mrna
4
plasmid group
4
mrna protein
4

Keyword Occurance

Similar Publications

Lower Cervical Cancer Risk Associated With HPV Vaccine.

Authors:
Carrie Printz

Cancer 2021 Apr;127(8):1171

View Article and Full-Text PDF
April 2021
Similar Publications

Incidence of cervical intraepithelial lesions and human papilloma virus infection in female renal transplant recipients.

Authors:
Idalia Parra-Avila María Luisa Jiménez-Santana Rosa Elena Barrón-Sánchez Rosa Areli Martínez-Gamboa Josefina Alberú Luis Eduardo Morales-Buenrostro Ma-Del-Carmen Cravioto

Transpl Infect Dis 2021 Apr 20:e13622. Epub 2021 Apr 20.

Departments of Reproductive Biology, Mexico.

Background: Female renal transplant recipients (RTR) are at high risk of human papillomavirus (HPV)-related anogenital premalignancies and cancer. The aim of this study was to estimate the incidence of cervical intraepithelial lesions (IL) and HPV infection, and their associated factors, in Mexican RTR.

Methods: This is a prospective cohort study conducted between January 2011 and December 2017. Read More

View Article and Full-Text PDF
April 2021
Similar Publications

A potential means to help the HPV vaccine penetrate the Japanese public while under the continued suspension of governmental recommendation.

Authors:
Natsuyo Ugumori Yutaka Ueda Asami Yagi Hazuki Abe Mayu Shiomi Satoshi Nakagawa Kosuke Hiramatsu Ai Miyoshi Eiji Kobayashi Toshihiro Kimura Tadashi Kimura Motowo Nabeta

Hum Vaccin Immunother 2021 Apr 20:1-6. Epub 2021 Apr 20.

Department of Obstetrics and Gynecology , Tsubaki Women's Clinic, Matsuyama, Japan.

In Japan, the government's recommendation for the HPV vaccine has been suspended for almost 8 years. A questionnaire survey was conducted in the Tsubaki Women's Clinic, Matsuyama, Japan, to examine responses of the mothers of girls eligible for HPV vaccine before and after their doctor provided them an informative leaflet explaining the need for cervical cancer prevention.Among the 53 mothers who admitted to imposing some preconditions before being willing to encourage their daughters' HPV vaccination, 21 (40%) mothers became more willing to vaccinate their daughters immediately after receiving the cervical cancer prevention linkage explanation provided by their doctor, and seven of the mothers (33%) even returned to the clinic to get their daughter vaccinated during our study period. Read More

View Article and Full-Text PDF
April 2021
Similar Publications

Real-world Effectiveness of Human Papillomavirus Vaccination Against Cervical Cancer.

Authors:
Susanne K Kjaer Christian Dehlendorff Federica Belmonte Louise Baandrup

J Natl Cancer Inst 2021 Apr 20. Epub 2021 Apr 20.

Unit of Virus, Lifestyle and Genes, Danish Cancer Society Research Center, Copenhagen, Denmark.

Background: The primary goal of human papillomavirus (HPV) vaccination is to reduce morbidity and mortality from HPV-associated disease, especially cervical cancer. We determined the real-world effectiveness of HPV vaccination against cervical cancer.

Methods: The study included women 17-30 years living in Denmark October 2006-December 2019. Read More

View Article and Full-Text PDF
April 2021
Similar Publications

Impact of reduced human papillomavirus vaccination coverage rates due to COVID-19 in the United States: A model based analysis.

Authors:
Vincent Daniels Kunal Saxena Craig Roberts Smita Kothari Shelby Corman Lixia Yao Linda Niccolai

Vaccine 2021 Apr 6. Epub 2021 Apr 6.

Department of Epidemiology of Microbial Diseases, Yale School of Public Health, PO Box 208034, 60 College Street, New Haven, CT 06520-8034, USA. Electronic address:

The coronavirus disease 2019 (COVID-19) pandemic has significantly affected utilization of preventative health care, including vaccines. We aimed to assess HPV vaccination rates during the pandemic, and conduct a simulation model-based analysis to estimate the impact of current coverage and future pandemic recovery scenarios on disease outcomes. The model population included females and males of all ages in the US. Read More

View Article and Full-Text PDF
April 2021
Similar Publications