Pubfacts - Scientific Publication Data
  • Categories
  • |
  • Journals
  • |
  • Authors
  • Login
  • Categories
  • Journals

Search Our Scientific Publications & Authors

Publications
  • Publications
  • Authors
find publications by category +
Translate page:

Identification and characterization of Pseudomonas aeruginosa derived bacteriocin for industrial applications.

Authors:
Ramasamy Lakshmanan Dharman Kalaimurugan Palaniappan Sivasankar Selvaraj Arokiyaraj Srinivasan Venkatesan

Int J Biol Macromol 2020 Dec 22;165(Pt B):2412-2418. Epub 2020 Oct 22.

Department of Environmental Science, School of Life Sciences, Periyar University, Salem 636011, Tamil Nadu, India. Electronic address:

Drug resistance has become a major threat due to the frequent use of commercial antibiotics and there is an urgent need to combat this problem. Having this in mind, the present research was aimed at developing a novel P. aeruginosa puBac bacteriocin molecule. The bacteriocin was purified by ammonium sulfate precipitation followed by Sepharose FF and Sephadex G15 column purification and the purified bacteriocin has been reported to have the molar mass of 43 kDa. The findings of the optimization showed that 3500 AU/mL of bacteriocin was obtained at 37 °C, 3410 AU/mL of bacteriocin at 6.5 pH and 3780 AU/mL of bacteriocin at 48 h of incubation time. In addition, 3863 AU/mL of bacteriocin activity was obtained with Tween-80 followed by 3789 AU/mL with a concentration of 2% NaCl and 4200 AU/mL for Fe. PuBac bacteriocin has been shown to have a significant effect on test pathogens. For example, E. coli was found to have 3.6 μM of MIC, followed by Staphylococcus sp. with 6.15 μM of MIC and Bacillus sp. with a 7.5 μM of MIC. The remarkable properties of bacteriocin suggest that it could be used in various pharmaceutical and food industries.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2020.10.126DOI Listing
December 2020

Publication Analysis

Top Keywords

bacteriocin
10
pubac bacteriocin
8
37 °c 3410 au/ml
4
bacteriocin 37 °c
4
optimization 3500 au/ml
4
3500 au/ml bacteriocin
4
3410 au/ml bacteriocin
4
bacteriocin 3780 au/ml
4
incubation time
4
time addition
4
48 h incubation
4
bacteriocin 48 h
4
3780 au/ml bacteriocin
4
findings optimization
4
mass 43 kda
4
sephadex g15
4
g15 column
4
sepharose sephadex
4
precipitation sepharose
4
sulfate precipitation
4

Keyword Occurance

Similar Publications

Pseudomonas syringae pv. Uses Distinct Modes of Stationary-Phase Persistence To Survive Bacteriocin and Streptomycin Treatments.

Authors:
Ravikumar R Patel Prem P Kandel Eboni Traverso Kevin L Hockett Lindsay R Triplett

mBio 2021 Apr 13;12(2). Epub 2021 Apr 13.

Department of Plant Pathology and Ecology, The Connecticut Agricultural Experiment Station, New Haven, Connecticut, USA

Antimicrobial treatment of bacteria often results in a small population of surviving tolerant cells, or persisters, that may contribute to recurrent infection. Antibiotic persisters are metabolically dormant, but the basis of their persistence in the presence of membrane-disrupting biological compounds is less well understood. We previously found that the model plant pathogen pv. Read More

View Article and Full-Text PDF
April 2021
Similar Publications

Antifungal potential against Sclerotinia sclerotiorum (Lib.) de Bary and plant growth promoting abilities of Bacillus isolates from canola (Brassica napus L.) roots.

Authors:
Igor Daniel Alves Ribeiro Evelise Bach Fernanda da Silva Moreira Aline Reis Müller Caroline Pinto Rangel Camila Mörschbächer Wilhelm Afonso Luis Barth Luciane Maria Pereira Passaglia

Microbiol Res 2021 Apr 2;248:126754. Epub 2021 Apr 2.

Departamento de Genética, Instituto de Biociências, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Caixa Postal 15.053, 91501-970, Porto Alegre, RS, Brazil. Electronic address:

Endophytic bacteria show important abilities in promoting plant growth and suppressing phytopathogens, being largely explored in agriculture as biofertilizers or biocontrol agents. Bacteria from canola roots were isolated and screened for different plant growth promotion (PGP) traits and biocontrol of Sclerotinia sclerotiorum. Thirty isolates belonging to Bacillus, Paenibacillus, Lysinibacillus, and Microbacterium genera were obtained. Read More

View Article and Full-Text PDF
April 2021
Similar Publications

The effect of dietary supplementation of sage plant extract and Enterocin M on the mucus in the the small intestine and caecum in rabbits.

Authors:
R Szabóová A Lauková R Herich L Tarabová Ľ Chrastinová Z Faixová Z Maková E Piešová

Pol J Vet Sci 2021 Mar;24(1):23-28

University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 040 81 Košice, Slovak Republic.

The aim of this study was to determine the beneficial effect of natural substances - enterocin M (Ent M; the proteinaceous substance produced by Enterococcus faecium CCM8558) and sage plant ( Salvia officinalis L.) extract on the production of mucus in the rabbits small intestine and caecum. Sixty four post-weaned rabbits (meat line M91) were divided into three experimental groups (EG - Ent M; SG - sage extract; ESG - combination Ent M with sage extract) and control group (CG). Read More

View Article and Full-Text PDF
March 2021
Similar Publications

Experimental Evolution of Interference Competition.

Authors:
Florien A Gorter Carolina Tabares-Mafla Rees Kassen Sijmen E Schoustra

Front Microbiol 2021 25;12:613450. Epub 2021 Mar 25.

Laboratory of Genetics, Wageningen University & Research, Wageningen, Netherlands.

The importance of interference competition, where individuals compete through antagonistic traits such as the production of toxins, has long been recognized by ecologists, yet understanding how these types of interactions evolve remains limited. Toxin production is thought to be beneficial when competing with a competitor. Here, we explore if antagonism can evolve by long-term selection of the toxin (pyocin) producing strain PAO1 in the presence (or absence) of one of three clinical isolates of the same species () over ten serial transfers. Read More

View Article and Full-Text PDF
March 2021
Similar Publications

Pangenome analyses of LuxS-coding genes and enzymatic repertoires in cocoa-related lactic acid bacteria.

Authors:
Almeida Ogg N Vitulo De Martinis Ecp G E Felis

Genomics 2021 Apr 8. Epub 2021 Apr 8.

University of Verona - Department of Biotechnology, Verona, Italy.

Lactobacillaceae presents potential for interspecific Quorum Sensing (QS) in spontaneous cocoa fermentation, correlated with high abundance of luxS. Three Brazilian isolates from cocoa fermentation were characterized by Whole Genome Sequencing and luxS gene was surveyed in their genomes, in comparison with public databases. They were classified as Lactiplantibacillus plantarum, Limosilactobacillus fermentum and Pediococcus acidilactici. Read More

View Article and Full-Text PDF
April 2021
Similar Publications
© 2021 PubFacts.
  • About PubFacts
  • Privacy Policy
  • Sitemap