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Role of rotifer () and () nauplii in the horizontal transmission of a natural nervous necrosis virus (NNV) reassortant strain to Senegalese sole () larvae.

Authors:
L Vázquez-Salgado J G Olveira C P Dopazo I Bandín

Vet Q 2020 Dec;40(1):205-214

Instituto de Acuicultura, Departamento de Microbiología y Parasitología, Universidade de Santiago de Compostela, Santiago de Compostela, Spain.

Background: Marine invertebrates are provided as a first feed for marine fish larvae because of their strict nutritional requirements, despite also being a potential source of infectious agents.

Aim: To assess horizontal transmission of a nervous necrosis virus reassortant strain (NNV) to sole larvae via and rotifers.

Materials And Methods: Rotifer () and () nauplii cultures were bath infected with a reassortant (RGNNV/SJNNV) NNV strain isolated from gilthead sea bream and viral internalisation was confirmed by IFA. Senegalese sole () larvae were fed on infected and disease signs and mortality were recorded. In addition, NNV viability was checked in cultures of either unfed invertebrates or invertebrates fed on phytoplankton and in the supernatant of microalgae cultures. All samples were tested by RT-qPCR and inoculation in cell culture.

Results: Both rotifers and internalised NNV. Experimental transmission to sole larvae was achieved using infected and subsequently 60% mortality was recorded. At 24 h post-infection, orally infected individuals contained 9.34 × 10 copies of viral RNA, whereas the bath infection yielded 2.05 × 10 RNA copies larvae. Viral presence in both invertebrates was detected up to 8 days post infection but viral load decreased over time. Feeding with microalgae decreased viral detection even more and microalgae supernatants were demonstrated to significantly affect NNV viability.

Conclusions: Our results demonstrate that both invertebrates can bioaccumulate NNV and that Senegalese sole larvae fed on infected might develop viral encephalopathy and retinopathy and high mortality.

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http://dx.doi.org/10.1080/01652176.2020.1810357DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7734120PMC
December 2020

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