Background: Marine invertebrates are provided as a first feed for marine fish larvae because of their strict nutritional requirements, despite also being a potential source of infectious agents.Aim: To assess horizontal transmission of a nervous necrosis virus reassortant strain (NNV) to sole larvae via and rotifers.Materials And Methods: Rotifer () and () nauplii cultures were bath infected with a reassortant (RGNNV/SJNNV) NNV strain isolated from gilthead sea bream and viral internalisation was confirmed by IFA.