Syst Biol Reprod Med 2020 Oct 18;66(5):300-310. Epub 2020 Aug 18.
Department of Ruminant Science, West Pomeranian University of Technology , Szczecin, Poland.
Artificial insemination has achieved a dynamic increase in genetic progress, and this is due to the improvement of sperm preservation technology. In recent years, a lot of attention has been paid to optimizing bull sperm storage environment and objectifying methods of sperm quality analysis. This review presents bull sperm preservation methods and ways to modify their storage environment. The main purpose of sperm preparation for artificial insemination is to obtain sperm with a high percentage of viable, motile sperm with normal morphology and low DNA fragmentation rates. Currently conducted experiments indicate the possibility of improving the quality of insemination doses produced using various components enriching common diluents. However, despite extensive research, no better results have been achieved than obtaining insemination doses with sperm viability that exceeds just over 60%. Obtaining a very good quality of frozen semen seems to be still unachievable today.