Chembiochem 2020 07 30;21(13):1868-1874. Epub 2020 Mar 30.
Istituto di Scienze e Tecnologie Chimiche "Giulio Natta" (SCITEC) - CNR, Via Mario Bianco, 9, 20131, Milano, Italy.
The non-hydrolytic ring opening of 1,2-epoxides in the presence of limonene epoxide hydrolases (LEHs) and different nucleophiles has been investigated. Lyophilized, wild-type LEHs were tested in selected water-saturated organic solvents in the presence of cyclohexene oxide as substrate and different alcohols, thiols and primary amines as nucleophiles. Although the LEHs retained an appreciable catalytic activity under different reaction conditions, formation of the desired 1,2-substituted cyclohexanols was not observed. Read More