Efforts to enhance the transformative potential of biofuels is an important step to achieving an environment-friendly and sustainable energy source. is an ideal third-generation biofuel agent due to its ability to produce lipids and desirable essential fatty acids. In this study, the impact of Nanofer 25s nanoscale zero-valent iron nanoparticles (nZVIs) on total lipid content and fatty acid composition of strains SF33 and B481 was investigated.