Biosci Biotechnol Biochem 2020 Sep 24;84(9):1913-1920. Epub 2020 May 24.
Bioprocess Technology, School of Industrial Technology, Universiti Sains Malaysia , Gelugor, Malaysia.
The aims of this study were to compare the effectiveness of different drying methods and to investigate the effects of adding a series of individual protectant such as skim milk, sucrose, maltodextrin, and corn starch for preserving FTDC 3081 cells during spray and freeze-drying and storage at different temperatures. Results showed a remarkable high survival rate of 70-80% immediately after spray- and freeze-drying in which the cell viability retained at the range of 10 to 10 CFU/mL. After a month of storage, maltodextrin showed higher protective ability on both spray- and freeze-dried cells as compared to other protective agents at 4°C, 25°C, and 40°C. A complete loss in viability of spray-dried FTDC 3081 was observed after a month at 40°C in the absence of protective agent.