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A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells.

Authors:
Haibin Xi Justin Langerman Shan Sabri Peggie Chien Courtney S Young Shahab Younesi Michael Hicks Karen Gonzalez Wakana Fujiwara Julia Marzi Simone Liebscher Melissa Spencer Ben Van Handel Denis Evseenko Katja Schenke-Layland Kathrin Plath April D Pyle

Cell Stem Cell 2020 07 11;27(1):158-176.e10. Epub 2020 May 11.

Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA; Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, CA, USA. Electronic address:

The developmental trajectory of human skeletal myogenesis and the transition between progenitor and stem cell states are unclear. We used single-cell RNA sequencing to profile human skeletal muscle tissues from embryonic, fetal, and postnatal stages. In silico, we identified myogenic as well as other cell types and constructed a "roadmap" of human skeletal muscle ontogeny across development. In a similar fashion, we also profiled the heterogeneous cell cultures generated from multiple human pluripotent stem cell (hPSC) myogenic differentiation protocols and mapped hPSC-derived myogenic progenitors to an embryonic-to-fetal transition period. We found differentially enriched biological processes and discovered co-regulated gene networks and transcription factors present at distinct myogenic stages. This work serves as a resource for advancing our knowledge of human myogenesis. It also provides a tool for a better understanding of hPSC-derived myogenic progenitors for translational applications in skeletal muscle-based regenerative medicine.

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http://dx.doi.org/10.1016/j.stem.2020.04.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7367475PMC
July 2020

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