J Biosci Bioeng 2020 Aug 4;130(2):195-199. Epub 2020 May 4.
Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan 32003, Taiwan; Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Taoyuan 32003, Taiwan. Electronic address:
Ectoine production using inexpensive and renewable biomass resources has attracted great interest among the researchers due to the low yields of ectoine in current fermentation approaches that complicate the large-scale production of ectoine. In this study, ectoine was produced from corn steep liquor (CSL) and soybean hydrolysate (SH) in replacement to yeast extract as the nitrogen sources for the fermentation process. To enhance the bacterial growth and ectoine production, biotin was added to the Halomonas salina fermentation media. In addition, the effects addition of surfactants such as Tween 80 and saponin on the ectoine production were also investigated. Results showed that both the CSL and SH can be used as the nitrogen source substitutes in the fermentation media. Higher amount of ectoine (1781.9 mg L) was produced in shake flask culture with SH-containing media as compared to CSL-containing media. A total of 2537.0 mg L of ectoine was produced at pH 7 when SH-containing media was applied in the 2 L batch fermentation. Moreover, highest amount of ectoine (1802.0 mg L) was recorded in the SH-containing shake flask culture with addition of 0.2 μm mL biotin. This study demonstrated the efficacy of industrial waste as the nutrient supplement for the fermentation of ectoine production.