Shift of HbF to HbA under Influence of SKA2 Gene; A Possible Link between Cortisol and Hematopoietic Maturation in Term and Preterm Newborns.

Endocr Metab Immune Disord Drug Targets 2020 May 3. Epub 2020 May 3.

Counseling and Reproductive Health Research Centre, Golestan University of Medical Sciences, Gorgan. Iran.

Background: We hypothesized that SKA2 gene can convert hemoglobin F to A leading to the maturity of the hematopoietic system by glucocorticoid hormone; so, the present study aimed to investigate the health outcome of newborns by using the effect of SKA2 gene on hematopoietic maturation.

Methods: At first, 142 samples were divided into term and preterm. After sampling from the umbilical cord blood, the expression of SKA2 genes and HbA and F were evaluated by quantitative RT-PCR. The blood gases were measured by Campact 3 device. Finally, cortisol level was measured by ELISA method and HbA and F levels were investigated by capillary electrophoresis.

Results: The blood gases and Apgar scores was more favorable in term newborns (P <0.001). Levels of protein/expression of HbF in newborns with Apgar score greater than 7 was lower than that of the newborns with Apgar score below 7 (P <0.001). Cortisol and HbA levels were considerably higher in term newborns compared to the preterm ones (P <0.001). In the preterm and term groups, SKA2 gene expression had a positive and significant relationship with cortisol and HbA levels as well as a negative relationship with HbF level. In the preterm group, a positive and significant relationship was observed between the expression of SKA2 and HbF genes.

Conclusion: The results revealed that the SKA2 gene affected on hematopoietic maturation in preterm and term newborns and the health outcome of newborns improved by increasing HbA level.

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Source
http://dx.doi.org/10.2174/1871530320666200504091354DOI Listing
May 2020

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