Organic matter reduces the amount of detectable environmental DNA in freshwater.

Ecol Evol 2020 Apr 21;10(8):3647-3654. Epub 2020 Mar 21.

Naturalis Biodiversity Center Leiden The Netherlands.

Environmental DNA (eDNA) is used for monitoring the occurrence of freshwater organisms. Various studies show a relation between the amount of eDNA detected and target organism abundance, thus providing a potential proxy for reconstructing population densities. However, environmental factors such as water temperature and microbial activity are known to affect the amount of eDNA present as well. In this study, we use controlled aquarium experiments using L. (Amphipoda) to investigate the relationship between the amount of detectable eDNA through time, pH, and levels of organic material. We found eDNA to degrade faster when organic material was added to the aquarium water, but that pH had no significant effect. We infer that eDNA contained inside cells and mitochondria is extra resilient against degradation, though this may not reflect actual presence of target species. These results indicate that, although estimation of population density might be possible using eDNA, measured eDNA concentration could, in the future, be corrected for local environmental conditions in order to ensure accurate comparisons.

Download full-text PDF

Source
http://dx.doi.org/10.1002/ece3.6123DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160167PMC
April 2020

Publication Analysis

Top Keywords

edna
8
environmental dna
8
organic material
8
amount detectable
8
amount edna
8
aquarium experiments
4
density edna
4
controlled aquarium
4
population density
4
experiments amphipoda
4
relationship amount
4
investigate relationship
4
estimation population
4
amphipoda investigate
4
detectable edna
4
well study
4
edna concentration
4
microbial activity
4
temperature microbial
4
degradation reflect
4

Altmetric Statistics

Similar Publications