Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcriptional control.

Genome Res 2020 04 6;30(4):515-527. Epub 2020 Apr 6.

Department of Cell Biology, Erasmus MC, 3015 GD Rotterdam, The Netherlands.

Cohesin is a ring-shaped multiprotein complex that is crucial for 3D genome organization and transcriptional regulation during differentiation and development. It also confers sister chromatid cohesion and facilitates DNA damage repair. Besides its core subunits SMC3, SMC1A, and RAD21, cohesin in somatic cells contains one of two orthologous STAG subunits, STAG1 or STAG2. How these variable subunits affect the function of the cohesin complex is still unclear. STAG1- and STAG2-cohesin were initially proposed to organize cohesion at telomeres and centromeres, respectively. Here, we uncover redundant and specific roles of STAG1 and STAG2 in gene regulation and chromatin looping using HCT116 cells with an auxin-inducible degron (AID) tag fused to either STAG1 or STAG2. Following rapid depletion of either subunit, we perform high-resolution Hi-C, gene expression, and sequential ChIP studies to show that STAG1 and STAG2 do not co-occupy individual binding sites and have distinct ways by which they affect looping and gene expression. These findings are further supported by single-molecule localizations via stochastic optical reconstruction microscopy (STORM) super-resolution imaging. Since somatic and congenital mutations of the STAG subunits are associated with cancer (STAG2) and intellectual disability syndromes with congenital abnormalities (STAG1 and STAG2), we verified STAG1-/STAG2-dependencies using human neural stem cells, hence highlighting their importance in particular disease contexts.

Download full-text PDF

Source
http://dx.doi.org/10.1101/gr.253211.119DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197483PMC
April 2020

Publication Analysis

Top Keywords

stag1 stag2
20
stag subunits
12
chromatin looping
8
specific roles
8
gene expression
8
redundant specific
8
stag2
6
stag1
5
subunits
5
stag2 gene
4
uncover redundant
4
roles stag1
4
subunits associated
4
telomeres centromeres
4
gene regulation
4
centromeres uncover
4
mutations stag
4
congenital mutations
4
auxin-inducible degron
4
cells auxin-inducible
4

Altmetric Statistics

Similar Publications