Development of qualitative and quantitative immunochromatographic strip test assay for rapid and simple detection of leucomalachite green residual in aquatic animals.

Food Chem 2020 Aug 14;320:126613. Epub 2020 Mar 14.

Thailand Institute of Scientific and Technological Research, Khlong Luang, Pathum Thani, 12120, Thailand.

A rapid and simple immunochromatographic strip test assay based on competitive format was developed for leucomalachite green (LMG) detection. LMG-bovine serum albumin and rabbit anti-sheep IgG were immobilized on nitrocellulose membrane for the test line and control line, respectively. Anti-LMG-colloidal gold conjugate was immobilized onto the conjugate pad. For qualitative detection, the cut-off limit of the strip test was determined at 2 µg/L by the naked eye. For quantitative analysis, the working range of the LMG detection was 0.7-2 µg/L with LOD at 0.28 µg/L. A one-step immunochromatographic strip test for LMG detection can be completed within 5 min without any incubation, washing and blocking steps. Analysis results of LMG in aquatic animals obtained from the immunochromatographic strip test were in good agreement with those realized from enzyme-link immunosorbent assay. The developed the immunochromatographic strip test offered rapid detection as a simple (one-step), cost-effective, instrument-free assay and no need for handling reagents.

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http://dx.doi.org/10.1016/j.foodchem.2020.126613DOI Listing
August 2020

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