Molecular mechanism of the anti-diabetic activity of an identified oligosaccharide from .

Res Pharm Sci 2020 Feb 20;15(1):36-47. Epub 2020 Feb 20.

Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, I.R. Iran.

Background And Purpose: Because of the high prevalence, diabetes is considered a global health threat. Hence, the need for effective, cheap, and comfortable therapies are highly felt. In previous study, a novel oligosaccharide with strong anti-diabetic activity in the crude extract of fruits, from the family, was identified. The present study was designed to ensure its efficacy using and studies.

Experimental Approach: Crude extract and its purified oligosaccharide were prepared from corresponding herb. Adult male Wistar rats were randomly divided into four groups of 10 each, as follows: group 1, healthy control rats given only sterile normal saline; group 2, diabetic control rats received sterile normal saline; group 3, diabetic rats treated with crude extract of Rosa canina (40% w/v) by oral gavage for 8 weeks; group 4, diabetic rats treated with purified oligosaccharide of Rosa canina (2 mg/kg) by oral gavage for 8 weeks. After treatment, body weight, fasting blood glucose, serum insulin levels and islet beta-cell repair and proliferation were investigated. The possible cytoprotective action of oligosaccharide was evaluated . The effect of oligosaccharide on apoptosis and insulin secretion in cell culture media were examined. Real-time PCR was used to determine the expression level of some glucose metabolism-related regulator genes.

Findings / Results: In the animal model of diabetes, the insulin levels were increased significantly due to the regeneration of beta-cells in the islands of langerhans by the purified oligosaccharide. cell apoptosis examination showed that high concentration of oligosaccharide increased cell death, while at low concentration protected cells from streptozotocin-induced apoptosis. Molecular study showed that the expression of and insulin production genes were increased, leading to increased expression of insulin-dependent genes such as and . On the other hand, the expression of the gene, which is related to the glucose transporter 2, was significantly reduced due to insulin concentrations.

Conclusion And Implications: The purified oligosaccharide from was a reliable anti-diabetic agent, which acted by increasing insulin production in beta-cells of the islands of Langerhans.

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Source
http://dx.doi.org/10.4103/1735-5362.278713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7053289PMC
February 2020

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