Bioactive Compounds in Lam. Leaves Inhibit the Pro-Inflammatory Mediators in Lipopolysaccharide-Induced Human Monocyte-Derived Macrophages.

Molecules 2020 Jan 2;25(1). Epub 2020 Jan 2.

Cellular and Molecular Immunology Research Unit, Faculty of Allied Health Sciences, Naresuan University, Phitsanulok 65000, Thailand.

(MO) is an important plant for traditional medicine. The present study aimed to identify the MO active phytochemical compounds for their ability against inflamed macrophages. An ethyl acetate extract fraction of MO was fractionation by flash column chromatography. Human macrophages were stimulated by Lipopolysaccharide and then treated with fractions of MO to examine their anti-inflammatory activity and cellular mechanism. The active fractions were analyzed by liquid chromatography with electrospray ionization quadrupole time-of-flight mass spectrometer (LC-ESI-QTOF-MS). MO treated cells showed a decreased production of pro-inflammatory mediator in response to lipopolysaccharide. This was evident at both mRNA and protein levels. The study revealed that MO suppressed mRNA expression of IL-1, IL-6, TNF-α, PTGS2, NF-κB (P50), and RelA. Furthermore, the extract effectively inhibited the expression of inflammatory mediators, including IL-6, TNF-α, and cyclooxygenase-2. Interestingly, the effect of MO inhibited phosphorylation of IκB-α and the ability to reduce expression of the nuclear factor (NF)-κB p65, suppressing its nuclear translocation. Moreover, LC-ESI-QTOF-MS analysis of the MO active fraction revealed seven compounds, namely 3,4-Methyleneazelaic acid, (2)-2-phenylmethoxybutane-1,4-diol, (2)-2-phenylmethoxybutane-1, 4-diol, γ-Diosphenol, 2,2,4,4-Tetramethyl-6-(1-oxobutyl)-1,3,5-cyclohexanetrione, 3-Hydroxy-β-ionone, and Tuberonic acid. Our findings highlight the ability of MO compounds to inhibit inflammation through regulation of the NF-κB pathway.

Download full-text PDF

Source
http://dx.doi.org/10.3390/molecules25010191DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6982846PMC
January 2020

Publication Analysis

Top Keywords

il-6 tnf-α
8
expression il-1
4
il-1 il-6
4
mrna expression
4
tnf-α ptgs2
4
suppressed mrna
4
levels study
4
study revealed
4
revealed suppressed
4
ptgs2 nf-κb
4
nf-κb p50
4
expression inflammatory
4
inflammatory mediators
4
mediators including
4
inhibited expression
4
effectively inhibited
4
p50 rela
4
rela extract
4
extract effectively
4
protein levels
4

Similar Publications