sp. nov., isolated from a marine sand.

Int J Syst Evol Microbiol 2020 Feb;70(2):909-914

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.

A Gram-stain-negative, aerobic, non-spore-forming, motile by gliding and rod-shaped bacterial strain, designated HSMS-1, was isolated from a marine sand collected from the Yellow Sea, Republic of Korea, and identified by a polyphasic taxonomic approach. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that HSMS-1 fell within the clade comprising the type strains of species of the genus . HSMS-1 exhibited 16S rRNA gene sequence similarity values of 99.0 and 98.7 % to the type strains of and and of 93.3-98.5 % to the type strains of the other species of the genus . The ANI and dDDH values between HSMS-1 and the type strains of , , , , and were 72.6-79.3 % and 17.4-22.2 %, respectively. Mean DNA-DNA relatedness value between HSMS-1 and the type strain of was 18 %. HSMS-1 contained MK-6 as the predominant menaquinone and iso-C, anteiso-C, iso-C 3-OH and iso-C as the major fatty acids. The major polar lipid of HSMS-1 was phosphatidylethanolamine. The DNA G+C content of HSMS-1 from genomic sequence data was 39.2 %. Distinguishing phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that HSMS-1 is separated from recognized species of the genus . On the basis of the data presented, strain HSMS-1 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HSMS-1(=KACC 19899=NBRC 113648).

Download full-text PDF

Source
http://dx.doi.org/10.1099/ijsem.0.003845DOI Listing
February 2020
Get 20% Off Journals at LWW.com

Similar Publications

Effects of 6 weeks treatment with dapagliflozin, a sodium-glucose co-transporter 2 inhibitor, on myocardial function and metabolism in patients with type 2 diabetes: a randomized placebo-controlled exploratory study.

Diabetes Obes Metab 2021 Feb 24. Epub 2021 Feb 24.

Turku PET Centre, University of Turku and Turku University Hospital, Turku, Finland.

Aims: To explore early effects of dapagliflozin on myocardial function and metabolism in patients with type 2 diabetes without heart failure.

Materials And Methods: Patients with type 2 diabetes on metformin treatment were randomized to double-blind 6-week placebo or dapagliflozin 10 mg daily treatment. Investigations included cardiac function and structure with myocardial resonance imaging (MRI); cardiac oxygen consumption, perfusion and efficiency with [ C]-acetate positron emission tomography (PET); and cardiac and hepatic fatty acid uptake with [ F]-FTHA PET, analyzed by ANCOVA as least square means with 95% confidence intervals. Read More

View Article and Full-Text PDF
February 2021

Development of an itraconazole resistance gene as a dominant selectable marker for transformation in Aspergillus oryzae and Aspergillus luchuensis.

Biosci Biotechnol Biochem 2021 Feb;85(3):722-727

United Graduate School of Agricultural Science, Kagoshima University, Korimoto, Kagoshima-shi, Kagoshima, Japan.

There are only a few combinations of antifungal drugs with known resistance marker genes in the Aspergillus species; therefore, the transformation of their wild-type strains is limited. In this study, to develop the novel dominant selectable marker for itraconazole, a fungal cell membrane synthesis inhibitor, we focused on Aspergillus luchuensis cyp51A (Alcyp51A), which encodes a 14-α-sterol demethylase related to the steroid synthesis pathway. We found that the G52R mutation in AlCyp51A and the replacement of the native promoter with a high-expression promoter contributed to itraconazole resistance in Aspergillus oryzae, designated as itraconazole resistant gene (itrA). Read More

View Article and Full-Text PDF
February 2021

Antiviral activity and mechanism of ESC-1GN from skin secretion of hylarana guentheri against influenza a virus.

J Biochem 2021 Feb 24. Epub 2021 Feb 24.

Guangdong Provincial Key Laboratory of New Drug Screening, Guangzhou Key laboratory of Drug Research for Emerging Virus Prevention and Treatment, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, 510515, China.

Development of new and effective anti-influenza drugs is critical for prophylaxis and treatment of influenza A virus infection. A wide range of amphibian skin secretions have been identified to show antiviral activity. Our previously reported ESC-1GN, a peptide from the skin secretion of Hylarana guentheri, displayed good antimicrobial and anti-inflammatory effects. Read More

View Article and Full-Text PDF
February 2021

Impact of the COVID-19 pandemic on the treatment of injuries during lockdown in Norway.

Scand J Public Health 2021 Feb 24:1403494821993725. Epub 2021 Feb 24.

Norwegian Institute of Public Health, Norway.

Aims: In order to prevent a major outbreak of COVID-19 disease in Norway, a series of lockdown measures was announced on 12 March 2020. The aim of the present paper was to describe the impact of this lockdown on the treatment of injuries.

Methods: We collected hospital data on injury diagnoses from a national emergency preparedness register established during the pandemic. Read More

View Article and Full-Text PDF
February 2021

Intraspecific nucleotide divergence in Saccharomycodes ludwigii, and proposal of Saccharomycodes pseudoludwigii sp. nov, a new apiculate yeast isolated from China.

Antonie Van Leeuwenhoek 2021 Feb 23. Epub 2021 Feb 23.

Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.

The six synonyms currently accepted under Saccharomycodes ludwigii were investigated for by phenotypic properties, however, the sequence diversity of the rRNA and protein coding genes have not yet been determined. Nine strains including the type strains of synonyms of S. ludwigii deposited in the CBS yeast collection, Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands, were analyzed using a multi-locus sequence analysis (MLSA) approach that included sequences of 18S ribosomal RNA (rRNA), the D1/D2 domains of the 26S rRNA, the ITS region (including the 5. Read More

View Article and Full-Text PDF
February 2021