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J Chem Phys 2022 Jun;156(24):244109

Laboratoire des Biomolécules, LBM, Département de Chimie, École Normale Supérieure, PSL University, Sorbonne Université, CNRS, 75005 Paris, France.

Polarization transfers are crucial building blocks in magnetic resonance experiments, i.e., they can be used to polarize insensitive nuclei and correlate nuclear spins in multidimensional nuclear magnetic resonance (NMR) spectroscopy. Read More

J Am Chem Soc 2022 Jul 1. Epub 2022 Jul 1.

Molecular Microbiology and Structural Biochemistry, UMR5086 CNRS/University of Lyon, 7, passage du Vercors, 69367 Lyon, France.

The detailed mechanism of ATP hydrolysis in ATP-binding cassette (ABC) transporters is still not fully understood. Here, we employed P solid-state NMR to probe the conformational changes and dynamics during the catalytic cycle by locking the multidrug ABC transporter BmrA in prehydrolytic, transition, and posthydrolytic states, using a combination of mutants and ATP analogues. The P spectra reveal that ATP binds strongly in the prehydrolytic state to both ATP-binding sites as inferred from the analysis of the nonhydrolytic E504A mutant. Read More

Nanoscale 2022 Jul 1. Epub 2022 Jul 1.

Department of Chemistry, University of Toronto, 80 St. George St, Toronto, Ontario, M5S 3H6, Canada.

Comprehensive Multiphase NMR (CMP-NMR) is a recently developed technique capable of simultaneously observing different phases - solutions, gels, and solids - while providing the chemical specificity of traditional NMR. With this new tool, the heterogeneous photocatalysis of phenol by titanium dioxide (P25 TiO) is re-examined to gain information about the occurrence of reaction at different regions between the catalyst and the solution. It was found that the proportion of phenol in different phases changes over the course of the photodegradation period. Read More

Front Chem 2022 14;10:898324. Epub 2022 Jun 14.

School of Life Sciences and Medicine, Shandong University of Technology, Zibo, China.

As rare-earth coordination polymers (CPs) have appreciable antimicrobial properties, ternary lanthanum CPs have been widely synthesized and investigated in recent years. Here, we report convenient, solvent-free reactions between the lanthanum salt and two ligands at mild temperatures that form ternary lanthanum nanoscale CPs with 10-gram-scale. The structural features and morphologies were characterized using a scanning electron microscope (SEM), Fourier transform infrared spectrometer (FT-IR), ultraviolet-visible (UV-Vis), X-ray diffractometer (XRD), X-ray Photoelectron Spectroscopy (XPS), Brunauer-Emmett-Teller (BET), elemental analysis, inductively coupled plasma mass spectrometry (ICP-MS), electrospray ionization mass spectrometry (ESI-MS), nuclear magnetic resonance (NMR), dynamic light scattering (DLS) and analyzer, and thermogravimetric and differential thermal analyzer (TG-DTA). Read More

Methods Mol Biol 2022 ;2507:201-221

Biotechnology and Cell Signalling, IMPReSs Protein Facility, UMR7242 CNRS-University of Strasbourg, Illkirch, France.

Membrane proteins (MPs) comprise about one-third of the human proteome, playing critical roles in many physiological processes and associated disorders. Consistently, they represent one of the largest classes of targets for the pharmaceutical industry. Their study at the molecular level is however particularly challenging, resulting in a severe lack of structural and dynamic information that is hindering their detailed functional characterization and the identification of novel potent drug candidates. Read More