Opposing peripheral fates of tissue-restricted self antigen-specific conventional and regulatory CD4 T cells.

Eur J Immunol 2020 01 14;50(1):63-72. Epub 2019 Oct 14.

Center for Immunology and Inflammatory Diseases, and Division of Pulmonary and Critical Care Medicine, Massachusetts General Hospital, and Harvard Medical School, Charlestown, MA, USA.

The development of self antigen-specific T cells is influenced by how the self antigen is expressed. Here, we created a mouse in which a model self antigen is conditionally expressed in different tissue environments. Using peptide:MHCII tetramer-based cell enrichment methods, we examined the development of corresponding endogenous self antigen-specific CD4 T cell populations. While ubiquitous self antigen expression resulted in efficient deletion of self antigen-specific T cells in the thymus, some tissue-restricted expression patterns resulted in partial deletion of the population in peripheral lymphoid organs. Deletion specifically affected Foxp3 conventional T cells (Tconv) with a bias towards high avidity TCR expressing cells in the case of thymic, but not peripheral deletion. In contrast, Foxp3 Treg exhibited elevated frequencies with increased TCR avidity. T cells surviving deletion were functionally impaired, with Tconv cells exhibiting more impairment than Tregs. Collectively, our results illustrate how postthymic recognition of tissue-restricted self antigens results in opposing developmental fates for Tconv and Treg cell subsets.

Download full-text PDF

Source
http://dx.doi.org/10.1002/eji.201948180DOI Listing
January 2020

Publication Analysis

Top Keywords

antigen-specific cells
8
cells
7
deletion
5
partial deletion
4
deletion population
4
cd4 cell
4
patterns partial
4
impairment tregs
4
population peripheral
4
peripheral lymphoid
4
organs deletion
4
deletion foxp3
4
lymphoid organs
4
exhibiting impairment
4
expression patterns
4
foxp3 conventional
4
tissue-restricted expression
4
antigen expression
4
expression efficient
4
cells surviving
4

Similar Publications