Multimodal molecular imaging of atherosclerosis: Nanoparticles functionalized with scFv fragments of an anti-αIIbβ3 antibody.

Nanomedicine 2019 11 9;22:102082. Epub 2019 Aug 9.

Centre de Résonance Magnétiques des Systèmes Biologiques, Bordeaux, France.. Electronic address:

Due to the wealth of actors involved in the development of atherosclerosis, molecular imaging based on the targeting of specific markers would substantiate the diagnosis of life-threatening atheroma plaques. To this end, TEG4 antibody is a promising candidate targeting the activated platelets (integrin αIIbβ3) highly represented within the plaque. In this study, scFv antibody fragments were used to functionalize multimodal imaging nanoparticles. This grafting was performed in a regio-selective way to preserve TEG4 activity and the avidity of the nanoparticles was studied with respect to the number of grafted antibodies. Subsequently, taking advantage of the nanoparticle bimodality, both near infrared fluorescence and magnetic resonance imaging of the atheroma plaque were performed in the ApoE mouse model. Here we describe the design of the targeted nanoparticles, and a quantification method for their detection in mice, both ex vivo and in vivo, highlighting their value as a potential diagnosis agent.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.nano.2019.102082DOI Listing
November 2019

Publication Analysis

Top Keywords

molecular imaging
8
teg4 activity
4
activity avidity
4
preserve teg4
4
avidity nanoparticles
4
regio-selective preserve
4
performed regio-selective
4
studied respect
4
grafted antibodies
4
antibodies subsequently
4
number grafted
4
respect number
4
grafting performed
4
nanoparticles studied
4
imaging nanoparticles
4
highly represented
4
represented plaque
4
αiibβ3 highly
4
integrin αiibβ3
4
platelets integrin
4

Similar Publications