A nanoflow cytometric strategy for sensitive ctDNA detection via magnetic separation and DNA self-assembly.

Anal Bioanal Chem 2019 Sep 15;411(23):6039-6047. Epub 2019 Jul 15.

CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, Jiangsu, China.

Circulating tumor DNA (ctDNA) is a tumor-derived fragmented DNA in the bloodstream that is not associated with cells. It has been greatly focused in the recent decade because of its potential clinical utility for liquid biopsies. Development of ctDNA analytical techniques with high sensitivity and cost-efficiency will undoubtedly promote the clinical spread of ctDNA testing. In this paper, we propose a novel flow cytometry-based ctDNA sensing strategy which combines enzyme-free amplification and magnetic separation. The target DNA is capable of triggering a hybridization chain reaction, producing a fluorescent long linear assembly of DNA, which can be further captured by magnetic beads to present fluorescent signals using flow cytometry. In comparison with some conventional methods, our strategy has the advantages of easy operation and cost-efficiency, and thereby shows a promising application in clinical diagnosis. Graphical abstract.

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http://dx.doi.org/10.1007/s00216-019-01985-xDOI Listing
September 2019

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References

(Supplied by CrossRef)

D Ogawara et al.
Thorac Cancer 2017

T Kishikawa et al.
World J Gastroenterol 2015

A Balmaseda et al.
P Natl Acad Sci USA 2017

EJH Wee et al.
Theranostics. 2016

YF Tan et al.
Biosens Bioelectron 2015

J Das et al.
J Am Chem Soc 2016

N Bellassai et al.
Anal Bioanal Chem 2016

L Qi et al.
Anal Chem 2017

AB Farimani et al.
ACS Appl Mater Interfaces 2017

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