Haemophilia 2019 Sep 11;25(5):e327-e330. Epub 2019 Jul 11.
Department of biologic Haematology, AP-HP, Hôpital Necker Paris, INSERM, UMR-S1176, University Paris Sud, University Paris-Saclay, Le Kremlin Bicêtre, France.
Allergy 2021 Apr 11. Epub 2021 Apr 11.
Division of Allergy and Immunology and Center for Pediatric Eosinophilic Disorders, Children's Hospital of Philadelphia.
Background: Eosinophilic esophagitis (EoE) is a T cell-mediated disease that iscaused by specific foods and results in esophageal dysfunction. Existing allergy testing modalities are not helpful when attempting to identify EoE-causal foods necessitating empiric food elimination and recurrent endoscopy.The goal of this study was to identify and compare allergen-specific immune features that can be assayed in a minimally-invasive manner to predict clinical food allergy in EoE. Read More
Anim Sci J 2021 Jan;92(1):e13542
Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Iwate University, Morioka City, Iwate, Japan.
The objectives of this study were to establish and characterize a homologous immunoassay for bovine chromogranin A (bCgA) and to profile plasma bCgA concentrations during early pregnancies. We synthesized oligopeptide corresponding to the amino acid sequence 341-355 of bCgA for immunizing rabbits and peptide corresponding to the amino acid sequence 336-365 of bCgA for both a biotinylated tracer and reference standards. Recombinant bCgA protein was also generated in Escherichia coli lysate. Read More
medRxiv 2021 Mar 1. Epub 2021 Mar 1.
Background: The novel coronavirus, SARS-CoV2 that causes COVID-19 has resulted in the death of more than 2.31 million people within the last year and yet no cure exists. Whereas passive immunization with COVID-19 convalescent plasma (CCP) provides a safe and viable option, selection of optimal units for therapy and lack of clear therapeutic benefit from transfusion remain as barriers to the use of CCP. Read More
Appl Spectrosc 2021 Mar 30:37028211002532. Epub 2021 Mar 30.
School of Chemistry and Molecular Biosciences, 1974The University of Queensland, St. Lucia, Australia.
Conventional methods for measuring the concentration of deuterium in body fluids are by either isotope ratio mass spectrometry or Fourier transform infrared transmission (FT-IR) spectroscopy. The latter method is often preferred as it is less expensive and time consuming; however, having a lower sensitivity means a larger sample volume is required. This study investigated an alternative FT-IR spectroscopic method, attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR), which has the potential to provide shorter analysis times while requiring smaller sample volumes. Read More
Clin Epigenetics 2021 Jan 21;13(1):14. Epub 2021 Jan 21.
Clinical Genomics Pty Ltd, North Ryde, NSW, Australia.
Background: Differentially-methylated regions (DMRs) are characteristic of colorectal cancer (CRC) and some occur more frequently than common mutations. This study aimed to evaluate the clinical utility of assaying circulating cell-free DNA for methylation in BCAT1, IKZF1 and IRF4 for detection of CRC.Methods: A multiplexed real-time PCR assay targeting DMRs in each of the three genes was developed. Read More