Defining early SIV replication and dissemination dynamics following vaginal transmission.

Sci Adv 2019 May 29;5(5):eaav7116. Epub 2019 May 29.

AIDS and Cancer Virus Program, Leidos Biomedical Research, Inc. Frederick National Laboratory for Cancer Research, Frederick, MD, USA.

Understanding HIV transmission is critical to guide the development of prophylactic interventions to prevent infection. We used a nonhuman primate (NHP) model with a synthetic swarm of sequence-tagged variants of SIVmac239 ("SIVmac239X") and scheduled necropsy during primary infection (days 3 to 14 after challenge) to study viral dynamics and host responses to the establishment and dissemination of infection following vaginal challenge. We demonstrate that local replication was initiated at multiple sites within the female genital tract (FGT), with each site having multiple viral variants. Local replication and spread in the FGT preceded lymphatic dissemination. Innate viral restriction factors were observed but appeared to follow viral replication and were ineffective at blocking initial viral establishment and dissemination. However, major delays were observed in time to dissemination in animals and among different viral variants within the same animal. It will be important to assess how phenotypic differences affect early viral dynamics.

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http://dx.doi.org/10.1126/sciadv.aav7116DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541462PMC
May 2019
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References

(Supplied by CrossRef)
Human immunodeficiency virus type 1 populations in blood and semen
Delwart et al.
J. Virol. 1998

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