High-Content Autophagy Analysis in iPSC-Derived Neurons Using Immunofluorescence.

Methods Mol Biol 2019 ;1994:165-174

MRC Laboratory for Molecular Cell Biology, University College London, London, UK.

Autophagy is the process by which cellular proteins and organelles are degraded and recycled and is essential to the survival of cells. Defective autophagic degradation has been linked to many neurodegenerative diseases and in particular lysosomal storage diseases. Here we describe a high-content assay to detect defects in the autophagy pathway in induced pluripotent stem cell-derived neurons. This assay utilizes immunofluorescence to stain autophagosomes and uses automated image analysis to measure changes in autophagosome levels in response to modulators of autophagy.

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http://dx.doi.org/10.1007/978-1-4939-9477-9_15DOI Listing
January 2019
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