Proteomics analysis of fetal growth restriction and taurine‑treated fetal growth restriction rat brain tissue by 2D DIGE and MALDI‑TOF/TOF MS analysis.

Int J Mol Med 2019 Jul 7;44(1):207-217. Epub 2019 May 7.

Department of Neonatology and NICU, Beijing Chaoyang District Maternal and Child Healthcare Hospital, Beijing 100101, P.R. China.

Fetal growth restriction (FGR) is caused by placental insufficiency and can lead to short and long‑term neurodevelopmental delays. Taurine, one of the most abundant amino acids in the brain, is critical for the normal growth and development of the nervous system; however, the mechanistic role of taurine in neural growth and development remains unknown. The present study investigated the role of taurine in FGR. Specifically, we explored the proteomic profiles of fetal rats at 6 h postpartum by two‑dimensional difference gel electrophoresis combined with matrix assisted laser desorption ionization‑time‑of‑flight (TOF)/TOF tandem mass spectrometry; the findings were verified via reverse transcription‑quantitative polymerase chain reaction. A total of 31 differentially expressed protein spots were selected. Among these, 31 were matched, including dihydropyrimidinase‑related protein 2 and , CRK and peroxiredoxin 2. Functional analysis using the Gene Ontology database and Ingenuity Pathway Analysis demonstrated that the differentially expressed proteins were mainly associated with neuronal differentiation, 'metabolic process', 'biological regulation' and developmental processes. The present study identified several proteins that were differentially expressed in rats with FGR in the presence or absence of taurine administration. The results of the present study suggest a potential role for taurine in the treatment and prevention of FGR.

Download full-text PDF

Source
http://dx.doi.org/10.3892/ijmm.2019.4182DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6559329PMC
July 2019
2 Reads

Article Mentions


Provided by Crossref Event Data
twitter
Twitter:
May 23, 2019, 7:36 am EST

Publication Analysis

Top Keywords

fetal growth
12
growth restriction
12
differentially expressed
12
role taurine
12
growth development
8
analysis fetal
8
growth
5
taurine
5
polymerase chain
4
chain reaction
4
reverse transcription‑quantitative
4
verified reverse
4
reaction total
4
transcription‑quantitative polymerase
4
protein spots
4
matched including
4
including dihydropyrimidinase‑related
4
dihydropyrimidinase‑related protein
4
selected matched
4
spots selected
4

Similar Publications