Intron retention as an alternative splice variant of the cattle ANGPTL6 gene.

Authors:
AiMin Li
AiMin Li
State Key Laboratory of Pollution Control and Resource Reuse
China
Hanfang Cai
Hanfang Cai
College of Animal Science and Technology
China
Chenge Zhang
Chenge Zhang
College of Animal Science and Technology
College Station | United States
Chuzhao Lei
Chuzhao Lei
College of Animal Science and Technology
China
Xianyong Lan
Xianyong Lan
College of Animal Science and Technology
China
Hong Chen
Hong Chen
Peking University People's Hospital
China

Gene 2019 Aug 15;709:17-24. Epub 2019 May 15.

Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, Shaanxi, Yangling 712100, PR China. Electronic address:

Angiopoietin-like protein 6, which is encoded by ANGPTL6 gene (also known as angiopoietin growth factor, AGF), has been extensively characterized with regard to its proposed functions as angiogenesis and energy metabolism. The present results showed the occurrence of alternative splicing by intron retention (IR) event in the bovine ANGPTL6 gene (bANGPTL6). By means of RT-PCR, TA clone and sequencing, we have shown that the bANGPTL6 gene has a splice variant generated by the retention of its partial intron 3. The computational analysis of the bANGPTL6 genomic sequence showed that its intron 3 has a high percentage of GC (62.31%) and a length of 199 nt, characteristics that have been associated with an IR event. The IR event does not interfere with the coding region as the bANGPTL6 prepropeptide is entirely coded in the third exon. Additionally, both the intronless (namely, bANGPTL6α) and intron-retaining (namely, bANGPTL6β) ANGPTL6 transcripts are constitutively co-expressed in the bovine liver. Further, the relative expression level of different variants in liver was tested by both semi-RT-PCR and RT-qPCR methods. The results suggested bANGPTL6β are significantly higher than bANGPTL6α. Overall, our findings will be helpful for studies on the molecular mechanism of IR events and the functions of ANGPTL6 gene. Specially, bANGPTL6β gene probably contributes to a new target for treatment of obesity and obesity-related diseases.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S03781119193049
Publisher Site
http://dx.doi.org/10.1016/j.gene.2019.05.031DOI Listing
August 2019
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