Single-Copy Knock-In Loci for Defined Gene Expression in .

G3 (Bethesda) 2019 07 9;9(7):2195-2198. Epub 2019 Jul 9.

Department of Genetics and Complex Diseases, Harvard T. H. Chan School of Public Health, Harvard University, Boston, Massachusetts 02115

We have generated a single-copy knock-in loci for defined gene expression (SKI LODGE) system to insert any DNA by CRISPR/Cas9 at defined safe harbors in the genome. Utilizing a single crRNA guide, which also acts as a Co-CRISPR enrichment marker, any DNA sequence can be introduced as a single copy, regulated by different tissue-specific promoters. The SKI LODGE system provides a fast, economical, and effective approach for generating single-copy ectopic transgenes in .

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Source
http://g3journal.org/lookup/doi/10.1534/g3.119.400314
Publisher Site
http://dx.doi.org/10.1534/g3.119.400314DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6643881PMC
July 2019

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