wound healing activity of 1-hydroxy-5,7-dimethoxy-2-naphthalene-carboxaldehyde (HDNC) and other isolates of marmelos L.: Enhances keratinocytes motility Wnt/β-catenin and RAS-ERK pathways.

Authors:
Dr. Mehnaz Kamal, PhD
Dr. Mehnaz Kamal, PhD
College of Pharmacy, Prince Sattam Bin Abdulaziz University
Assistant Professor
Medicinal chemistry
Al Kharj, Riyadh | Saudi Arabia

Saudi Pharm J 2019 May 29;27(4):532-539. Epub 2019 Jan 29.

Department of Pharmacology, College of Medicine, Al Imam Mohammad Ibn Saud Islamic University (IMSIU), P.O. Box 11623, Riyadh 11544, Saudi Arabia.

Wound healing is a complex process in which injured skin and tissues repaired by interaction of a complex cascade of cellular events that generates resurfacing, reconstitution and restoration of the tensile strength of injured skin. It follows β-catenin, extracellular signal regulated kinase (ERK) and Akt signaling pathways. Aegle marmelos L., generally known as bael is found to act as anti-inflammatory, antioxidant and anti-ulcer agent. Furthermore, studies have demonstrated that this Indian traditional medicinal plant, flower extract (AMF) was used for wound injury. Henceforth, the current study was investigated to ascertain the effect of its active constituents wound healing with mechanism involve in migration of cells and activation of β-catenin in keratinocytes, inhibition of PGE in macrophages and production of collagen in fibroblasts. We have taken full thickness wound of rats and applied AMF for 2 weeks. Cutaneous wound healing activity was performed using HaCaT keratinocytes, Hs68 dermal fibroblasts and RAW264.7 macrophages to determine cell viability, nitric oxide production, collagen expression, cell migration and β-catenin activation. Results shows that AMF treated rats demonstrated reduced wound size and epithelisation was improved, involved in keratinocytes migration by regulation of Akt signaling, beta-catenin and extracellular signal-regulated kinase (ERK) pathways. AMF and its active constituent's increased mRNA expression, inhibited nitric oxide, PGE release, mRNA expression of mediators in RAW 264.7 macrophages and enhances the motility of HaCaT keratinocytes wound healing of rats.

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Source
http://dx.doi.org/10.1016/j.jsps.2019.01.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6488852PMC
May 2019
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