A new role for RU486 (mifepristone): it protects sperm from premature capacitation during cryopreservation in buffalo.

Authors:
Dr.  Jasmer Dalal, PhD
Dr. Jasmer Dalal, PhD
Central Institute of Research on Buffalo
Dr.
Veterinary Gynaecology & Obstetrics
Hisar, Haryana | India
Pradeep Kumar
Pradeep Kumar
School of Therapeutic Sciences
South Africa
Rasika Rajendran
Rasika Rajendran
ICAR-Central Institute for Research on Buffaloes
Hisar | India
Dharmendra Kumar
Dharmendra Kumar
ICAR-Central Institute for Research on Buffaloes
United States

Sci Rep 2019 Apr 30;9(1):6712. Epub 2019 Apr 30.

Animal Physiology and Reproduction Division, ICAR- Central Institute for Research on Buffaloes, Hisar, 125001, Haryana, India.

The objective of this study was to determine the mechanism by which RU 486 (mifepristone) protects sperm to undergo premature capacitation during cryopreservation. For this, semen ejaculate (n = 20) was divided into four equal fractions and diluted using egg yolk-based extender supplemented with different concentrations of RU 486 (0, 5, 10 and 20 µM) and cryopreserved. We found that RU 486 did not impair the post-thaw sperm kinetics and motility but prevented cholesterol efflux, calcium influx, and protected CatSper channels during cryopreservation. The RU 486 protected sperm from premature capacitation which was confirmed by intracellular calcium level, expression of tyrosine phosphorylated proteins (75 and 80 kDa) and CTC (chlortetracycline) assay. Furthermore, antioxidant ability of RU 486 was reflected by the ferric reducing ability, lower production of sperm malondialdehyde and intracellular reactive oxygen species. Also, we demonstrated that RU 486 treated sperm underwent normal capacitation, zona pellucida binding and zygote cleavage indicating normal fertilizing ability of sperm. In conclusion, we report a new role of RU 486 in protecting buffalo sperm from premature capacitation during cryopreservation.

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Source
http://www.nature.com/articles/s41598-019-43038-4
Publisher Site
http://dx.doi.org/10.1038/s41598-019-43038-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6491824PMC
April 2019
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