Common Bile Duct Ligation as Model for Secondary Biliary Cirrhosis.

Authors:
Hans Van Vlierberghe
Hans Van Vlierberghe
Ghent University Hospital
Belgium
Lindsey Devisscher
Lindsey Devisscher
University of Ghent
Belgium

Methods Mol Biol 2019 ;1981:237-247

Basic and Applied Medical Sciences, Ghent University, Ghent, Belgium.

Cholestatic liver disease covers a range of biliary disorders marked by an impaired bile duct flow. Various conditions can result in bile obstruction including choledocholithiasis, surgical trauma, and autoimmune disorders. Cholestatic liver disease can be mild but generally progresses to more severe conditions with increased hepatobiliary injury, cholangitis, and ultimately liver fibrosis and cirrhosis. An extensively used experimental model to investigate the pathophysiology of biliary cirrhosis and potential novel therapies is the common bile duct ligation in mice and rats. Common bile duct ligation induces the different stages of cholestatic-induced liver disease being cholestasis, subsequently accompanied by inflammation and finally liver fibrosis and cirrhosis. In this protocol, an outline of the surgical procedures to conduct common bile duct ligation in mice is provided. The major steps include the isolation of the common bile duct, followed by ligation and dissection.

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http://dx.doi.org/10.1007/978-1-4939-9420-5_15DOI Listing
January 2019
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