An Inulin-Specific Lectin with Anti-HIV-1 Reverse Transcriptase, Antiproliferative, and Mitogenic Activities from the Edible Mushroom .

Biomed Res Int 2019 19;2019:1341370. Epub 2019 Mar 19.

State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100193, China.

A novel lectin (ABL) was purified from the dried fruiting bodies of . An efficient 3-step purification protocol involved two consecutive steps of ion exchange chromatography on Q-Sepharose and SP-Sepharose and gel filtration by FPLC on Superdex 75. ABL is a monomeric protein with the molecular mass of 27.6 kDa, which is different from other lectins from genus . Its N-terminal amino acid sequence is EYTISIRVYQTNPKGFNRPV which is unique and sharing considerably high similarity of other mushroom lectins. The hemagglutinating activity of the lectin was inhibited by inulin. Based on hemagglutination tests, ABL prefers rabbit, human type A, and AB erythrocytes to human type B and O erythrocytes. The lectin inhibits the activity of HIV-1 reverse transcriptase and the proliferation of leukemia cell (L1210) with an IC value of 4.69 and 4.97 M, respectively. Furthermore, ABL demonstrates the highest mitogenic activity with a response of 24177.7 ± 940.6 [H-methyl] thymidine counts per minute (CPM) at a concentration of 0.91 M.

Download full-text PDF

Source
https://www.hindawi.com/journals/bmri/2019/1341370/
Publisher Site
http://dx.doi.org/10.1155/2019/1341370DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444243PMC

Still can't find the full text of the article?

We can help you send a request to the authors directly.
March 2019
5 Reads

Publication Analysis

Top Keywords

type erythrocytes
8
human type
8
reverse transcriptase
8
cell l1210
4
lectins genus
4
kda lectins
4
276 kda
4
mass 276
4
genus n-terminal
4
leukemia cell
4
amino acid
4
proliferation leukemia
4
n-terminal amino
4
l1210 469
4
sequence eytisirvyqtnpkgfnrpv
4
acid sequence
4
protein molecular
4
filtration fplc
4
fplc superdex
4
gel filtration
4

References

(Supplied by CrossRef)

Similar Publications