Humoral Response to the HIV-1 Envelope V2 Region in a Thai Early Acute Infection Cohort.

Authors:
Hung V Trinh
Hung V Trinh
University of Zurich
Switzerland
Neelakshi Gohain
Neelakshi Gohain
Institute of Human Virology
Baltimore | United States
Christopher Hamlin
Christopher Hamlin
University of Notre Dame
Hongshuo Song
Hongshuo Song
Peking University Health Science Center
China
Eric Sanders-Buell
Eric Sanders-Buell
Walter Reed Army Institute of Research
United States
Leigh A Eller
Leigh A Eller
Walter Reed Army Institute of Research

Cells 2019 Apr 19;8(4). Epub 2019 Apr 19.

U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.

Reduced risk of HIV-1 infection correlated with antibody responses to the envelope variable 1 and 2 regions in the RV144 vaccine trial. To understand the relationship between antibody responses, V2 sequence, and structure, plasma samples (n = 16) from an early acute HIV-1 infection cohort from Thailand infected with CRF01_AE strain were analyzed for binding to V2 peptides by surface plasmon resonance. Five participants with a range of V2 binding responses at week 24 post-infection were further analyzed against a set of four overlapping V2 peptides that were designed based on envelope single-genome amplification. Antibody responses that were relatively consistent over the four segments of the V2 region or a focused response to the C-strand (residues 165-186) of the V2 region were observed. Viral escape in the V2 region resulted in significantly reduced antibody binding. Structural modeling indicated that the C-strand and the sites of viral variation were highly accessible in the open conformation of the HIV-1 Env trimer. V2 residues, 165-186 are preferentially targeted during acute infection. Residues 169-184 were also preferentially targeted by the protective immune response in the RV144 trial, thus emphasizing the importance of these residues for vaccine design.

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Source
http://dx.doi.org/10.3390/cells8040365DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6523213PMC
April 2019
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