Rapid profiling of triglycerides in human breast milk using liquid extraction surface analysis Fourier transform mass spectrometry reveals new very long chain fatty acids and differences within individuals.

Authors:
Albert Koulman
Albert Koulman
University of Groningen
Netherlands
Samuel Furse
Samuel Furse
College Lane
United Kingdom
Mark Baumert
Mark Baumert
Advion
Ithaca | United States
Gail Goldberg
Gail Goldberg
Elsie Widdowson Laboratory
United Kingdom
Les Bluck
Les Bluck
University of Cambridge
United Kingdom

Rapid Commun Mass Spectrom 2019 Aug;33(15):1267-1276

The Gambia Physiological Modelling of Metabolic Risk, MRC HNR, Cambridge, CB1 9NL, UK.

Rationale: We describe a novel method for preparing milk samples and profiling their triglyceride (TG) fractions. This method was used to explore how the TG profile of milk modulates as lactation progresses and how the TG profile differs between breasts.

Methods: Fresh milk was spotted onto Whatman filter paper and air-dried. Liquid Extraction Surface Analysis coupled to Fourier Transform Mass Spectrometry (LESA-MS) was adapted for molecular profiling. Collision-Induced Dissociation (CID) was used to profile fatty acid residues.

Results: LESA-MS produced the relative abundances of all isobaric TGs described and showed that mammary glands within one individual can produce a different profile of TGs. CID was used to uncover the configuration of isobaric triglycerides, indicating the relative amounts of the fatty acids contributing to that triglyceride's mass. This also indicated the presence of very long chain fatty acids (C26:0 and C26:1) that have not been reported before in human breast milk.

Conclusions: We conclude that spotting on paper and the use of LESA-MS and CID on milk spots is not only a means for analysing milk in unprecedented detail for this preparation time, but is also amenable to conditions in which collecting and storing fresh milk samples for detailed profiling is prohibitively difficult.

Download full-text PDF

Source
http://dx.doi.org/10.1002/rcm.8465DOI Listing

Still can't find the full text of the article?

We can help you send a request to the authors directly.
August 2019
3 Reads

Article Mentions


Provided by Crossref Event Data
twitter
Twitter:
April 23, 2019, 2:48 am EST
twitter
Twitter:
April 23, 2019, 1:52 am EST

Publication Analysis

Top Keywords

fatty acids
12
fourier transform
8
surface analysis
8
mass spectrometry
8
extraction surface
8
long chain
8
milk samples
8
chain fatty
8
liquid extraction
8
transform mass
8
human breast
8
fresh milk
8
milk
7
mammary glands
4
glands individual
4
individual produce
4
analysis coupled
4
paper air-dried
4
described mammary
4
tgs described
4

References

(Supplied by CrossRef)

Goldberg GR et al.
2008
Lipids of human milk and infant formulas: A review
Jensen RG et al.
Am J Clin Nutr 1978
Molecular weight distributions of milk fat triglycerides from seven species
Breckenridge WC et al.
J Lipid Res 1967
Specific distribution of short‐chain fatty acids in molecular distillates of bovine milk fat
Breckenridge WC et al.
J Lipid Res 1968

Similar Publications