Differential response of human blood leukocytes to brushite, monetite, and calcium polyphosphate biomaterials.

Authors:
Noah Fine
Noah Fine
University of Toronto
Canada
Zeeshan Sheikh
Zeeshan Sheikh
University of Toronto
Dr
Toronto, Ontario | Canada
Morvarid Oveisi
Morvarid Oveisi
Islamic Azad University of Pharmaceutical Sciences
Iran
Youxin Hu
Youxin Hu
Institute of Biomaterials and Biomedical Engineering
Canada
Robert Pilliar
Robert Pilliar
University of Toronto
Canada
Marc Grynpas
Marc Grynpas
University of Toronto
Canada

J Biomed Mater Res B Appl Biomater 2019 Apr 22. Epub 2019 Apr 22.

Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada.

Calcium phosphate-based biomaterials are extensively used for bone replacement and regeneration in orthopedic, dental, and maxillofacial surgical applications. The injury induced by surgical implantation of bone replacement graft materials initiates a cascade of host responses, starting with blood-biomaterial contact, protein adsorption on the material surface, blood coagulation, and leukocyte responses. During the initial acute inflammatory response, polymorphonuclear neutrophils (PMNs) and monocytes, abundant circulating leukocytes of the myeloid lineage, are recruited to the site of inflammation. In addition to responding to pathogenic challenges, these cells respond to particulate substances within the body including crystals of monosodium urate (MSU). Host responses toward grafts impact short- and long-term success in tissue engineering and regenerative applications. Although multinucleated osteoclasts, formed by monocyte/macrophage fusion, are generally thought to be responsible for resorption of implant biomaterials, the ability of different biomaterials to trigger PMNs, which are invariably present at the early stages after implant surgery, and are abundant in the oral cavity, has never been tested. In this article, we present analysis of the response of human blood-derived PMNs and monocytes toward brushite, monetite, and calcium polyphosphate (CPP) biomaterial substrates and compare this to the response to MSU crystals, the latter serving as a positive control. Employing multicolor flow cytometry to look at PMN and monocyte cell surface markers of activation to gauge the response to different biomaterials, we found that both types of myeloid cells are highly activated after exposure to brushite, monetite, and MSU but not CPP. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res B Part B, 2019.

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http://dx.doi.org/10.1002/jbm.b.34385DOI Listing

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April 2019
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