Cell wall mannoprotein of Candida albicans polarizes macrophages and affects proliferation and apoptosis through activation of the Akt signal pathway.

Authors:
Yu-Jing Zhang
Yu-Jing Zhang
Mailman School of Public Health of Columbia University
United States
Rui-qun Qi
Rui-qun Qi
First Hospital of China Medical University
China
Hong-Duo Chen
Hong-Duo Chen
No. 1 Hospital of China Medical University
China
Xing-Hua Gao
Xing-Hua Gao
Ji'nan Central Hospital
Jinan Shi | China

Int Immunopharmacol 2019 Apr 17;72:308-321. Epub 2019 Apr 17.

Department of Dermatology, The First Hospital of China Medical University, Shenyang 110001, PR China; Key Lab of Dermatology, Ministry of Education and Public Health, National joint Engineering Research Center for Theranostics of Immunological Skin Diseases, Shenyang 110001, PR China. Electronic address:

Candida albicans is a commensal fungus that associates with human hosts. Under normal circumstances this interaction does not produce any severe life-threatening disease, as macrophages of the innate immune system will result in its clearance. However, disorders may arise in immunosuppressed individuals. To understand the bioactivity of Candida albicans cell wall polysaccharides, which represent an important component of its function, mannoprotein from this fungus was extracted, purified and analyzed. Mannoprotein with α-(1,2) and α-(1,6) linkages was investigated with use of HPLC and NMR. Co-incubation of mannoprotein with macrophages resulted in a mannoprotein with the potential to polarize macrophages to M1 and promote phagocytosis/microbial killing ability thus increasing the clearance of pathogens through Akt2. Moreover, mannoprotein within the cell wall promoted cell proliferation and inhibited apoptosis by activation of the Akt signaling pathway. Collectively, α-(1,6)(1,2)-mannoprotein, one of the five polysaccharides extracted from the cell wall of Candida albicans, demonstrates immune-enhancing effects by activation of the Akt signaling pathway. These findings provide important new insights into the biological effects of polysaccharides on macrophages. Such information can then serve as the foundation for the development of novel anti-fungal medications.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S15675769193058
Publisher Site
http://dx.doi.org/10.1016/j.intimp.2019.03.032DOI Listing
April 2019
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