Oridonin inhibits LPS-induced inflammation in human gingival fibroblasts by activating PPARγ.

Authors:
Weili Xie
Weili Xie
School of Materials Science and Engineering
Yu Sun
Yu Sun
College of Life Sciences
Provo | United States

Int Immunopharmacol 2019 Apr 17;72:301-307. Epub 2019 Apr 17.

First Clinical Hospital of Harbin Medical University, Harbin 150001, China. Electronic address:

Oridonin, the major terpene isolated from Rabdosia rubescens, has been used as dietary supplement. Recently, it has been known to exhibit anti-inflammatory effect. This study we employed an in vitro model of LPS-stimulated human gingival fibroblasts to investigate the anti-inflammatory effects and mechanism of oridonin. Oridonin (10-30 μg/mL) was administrated 1 h before LPS treatment. The results showed that oridonin significantly inhibited inflammatory mediators PGE, NO, IL-6, and IL-8 production. Immunoblotting experiments revealed that oridonin reduced the expression of phosphorylation levels of NF-κB p65 and IκBα. Furthermore, the expression of PPARγ was up-regulated by the treatment of oridonin. Further studies showed that PPARγ inhibitor GW9662 could reverse the inhibition of oridonin on PGE, NO, IL-6, and IL-8 production. In conclusion, oridonin inhibited LPS-induced microglia activation through activating PPARγ.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S15675769183066
Publisher Site
http://dx.doi.org/10.1016/j.intimp.2019.04.006DOI Listing
April 2019
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