Curcumin attenuates collagen-induced rat arthritis via anti-inflammatory and apoptotic effects.

Authors:
Qirui Wang
Qirui Wang
Southern Medical University
China
Chanqi Ye
Chanqi Ye
Medical College of Nanchang University
Shukun Sun
Shukun Sun
Medical College of Nanchang University
Rong Li
Rong Li
Center for Cell Dynamics
United States
Xiaojian Shi
Xiaojian Shi
Jinan University
China
Shuai Wang
Shuai Wang
College of Veterinary Medicine
College Station | United States
Xiaoping Zeng
Xiaoping Zeng
Shenzhen Beike Cell Engineering Research Institute
China

Int Immunopharmacol 2019 Apr 17;72:292-300. Epub 2019 Apr 17.

Department of Immunology, Medical College of Nanchang University, Nanchang 330000, China. Electronic address:

Curcumin is a natural herbal product that has been popularly used to treat autoimmune diseases in China; however, its effects on rheumatoid arthritis and its mechanism are not clear. The main purposes of this study are to explore the therapeutic effects of curcumin on collagen-induced arthritis (CIA) rats and the pharmacological mechanism. In the present study, CIA rats were established by injecting bovine type II collagen. Curcumin and methotrexate were then orally administered daily, and the swelling degree of the hind limb joints was scored every two days. Histopathological changes were observed by hematoxylin-eosin staining. The levels of cytokines (TNF-α, IL-1β, IL-17 and TGF-β) were detected by radioimmunoassay, while the expression of IκBα and COX-2 was detected by Western blot. In addition, cell viability was detected by CCK-8 assay, and the effect of curcumin on macrophage apoptosis was detected by flow cytometry and TUNEL assay. The results indicated that in vivo curcumin attenuated the degree of joint swelling of rats and the further development of joint histopathology. Moreover, it downregulated the levels of cytokines. In vitro curcumin inhibited the degradation of IκBα and reduced the production of COX-2 in LPS-induced inflammatory RAW264.7 cells. Importantly, curcumin significantly induced macrophage apoptosis. In conclusion, in this study, we have demonstrated that curcumin exerts therapeutic effects on arthritis in CIA rats and has a strong pharmacological activity on reducing the inflammatory response in macrophages. Its mechanism may be related to the inhibition of the NF-κB signaling pathway and the promotion of macrophage apoptosis.

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S15675769193079
Publisher Site
http://dx.doi.org/10.1016/j.intimp.2019.04.027DOI Listing
April 2019
7 Reads

Publication Analysis

Top Keywords

cia rats
12
macrophage apoptosis
12
curcumin
9
effects curcumin
8
therapeutic effects
8
levels cytokines
8
arthritis cia
8
apoptosis detected
4
curcumin macrophage
4
assay curcumin
4
cell viability
4
viability detected
4
detected cck-8
4
cck-8 assay
4
cytometry tunel
4
vivo curcumin
4
curcumin attenuated
4
attenuated degree
4
indicated vivo
4
assay indicated
4

Similar Publications