Imiquimod enhances DNFB mediated contact hypersensitivity in mice.

Authors:
Shurong Ren
Shurong Ren
Medical College of Qingdao University
China
Yanli Zhang
Yanli Zhang
Nanjing Agricultural University
China
Ms. Bei Zhang
Ms. Bei Zhang
Duke-NUS Medical School
MD. Candidate
United states
Xue Dong
Xue Dong
School of Pharmaceutical Sciences
China
Wendi Zhang
Wendi Zhang
Guangdong Provincial Key Laboratory of Gastroenterology
China

Int Immunopharmacol 2019 Apr 17;72:284-291. Epub 2019 Apr 17.

Department of Immunology, Basic Medical College of Qingdao University, Qingdao 266071, China.

Imiquimod (Imiq) is a synthetic imizoquinoline compound which can act on Toll-like receptor (TLR)7 and transduce signals involved in cell activation. We investigated the role of Imiq on contact hypersensitivity (CHS) and explored the potential mechanisms of mast cells involved in the process. Topical application of Imiq cream augmented DNFB mediated CHS in C57BL/6 mice. Imiq application induced skin inflammation and increased the number of dendritic cells (DCs) in the draining lymph nodes (DLNs). The splenic cell proliferation to DNBS in DNFB and Imiq treated mice was greater than that in mice of DNFB treatment alone. Peritoneal cell-derived mast cells (PCMCs) expressed TLR7 mRNA. The results from toluidine blue staining for mast cells and histamine detection indicated that Imiq alone did not induce mast cell degranulation while Imiq plus DNFB significantly induced mast cell degranulation. Cromolyn, pyrilamine and cimetidine attenuated CHS reaction induced by Imiq. Our findings suggest that Imiq could augment the intensity of CHS reaction. The mechanisms underlying the effect may relate to histamine release by mast cells and induction of DC homing to DLNs. Blocking histamine action in early time of allergen contact is beneficial to the alleviation of CHS.

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Source
http://dx.doi.org/10.1016/j.intimp.2019.04.025DOI Listing
April 2019
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