Discovery of cycloneolignan enantiomers from Isatis indigotica Fortune with neuroprotective effects against MPP-induced SH-SY5Y cell injury.

Authors:
Wei Hong
Wei Hong
Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education
Guangzhou Shi | China
Xiao-yu Song
Xiao-yu Song
Beijing Research Center for Information Technology in Agriculture
Li-li Lou
Li-li Lou
Peking University School and Hospital of Stomatology
China
Le Zhou
Le Zhou
Northwest A&F University
China
Guo-Dong Yao
Guo-Dong Yao
The Affiliated Tumor Hospital of Harbin Medical University
China
Bin Lin
Bin Lin
175th Hospital of PLA
China

Bioorg Chem 2019 Apr 15;88:102926. Epub 2019 Apr 15.

School of Traditional Chinese Materia Medica, Key Laboratory of Computational Chemistry-Based Natural Antitumor Drug Research & Development, Liaoning Province, Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China. Electronic address:

A pair of new cycloneolignan enantiomers (1a and 1b) were isolated from the leaves of Isatis indigotica Fortune. Their structures were elucidated by extensive spectroscopic data analysis, including 1D and 2D NMR, HRESIMS, MS/MS analysis, together with theoretical electronic circular dichroism (ECD) calculations. Compounds 1a and 1b were then evaluated for their neuroprotective effects against MPP-induced SH-SY5Y cell injury. As a result, compounds 1a (77.64%) and 1b (78.62%) exhibited moderate neuroprotective activity at the concentration of 12.5 µM compared with that of MPP treated group (62.00% at 1 mM) by MTT assay. Furthermore, Annexin V-FITC/PI analysis showed that apoptosis ratios of 1a and 1b were reduced to 10.99% and 9.31%, respectively.

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http://dx.doi.org/10.1016/j.bioorg.2019.102926DOI Listing
April 2019
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