Metabolic mechanisms of Fuzheng-Huayu formula against liver fibrosis in rats.

Authors:
Ya-Nan Song
Ya-Nan Song
Shanghai University of TCM
China
Rong Wu
Rong Wu
Shengjing Hospital of China Medical University
China
Yongyu Zhang
Yongyu Zhang
State Key Laboratory of Marine Environmental Science
Jing-hua Peng
Jing-hua Peng
Shuguang Hospital
China
Yi-Yang Hu
Yi-Yang Hu
Fourth Military Medical University
China
Shi-bing Su
Shi-bing Su
Research Center for Traditional Chinese Medicine Complexity System
China

J Ethnopharmacol 2019 Jun 17;238:111888. Epub 2019 Apr 17.

Research Center for Complex System of Traditional Chinese Medicine, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. Electronic address:

Ethnopharmacological Relevance: Fuzheng-Huayu formula (FZHY) is traditionally used to treat liver fibrosis in clinic. The study was conducted to investigate the metabolic mechanisms of FZHY against liver fibrosis in rats.

Materials And Methods: Rats with CCl -induced liver fibrosis were treated with FZHY and its components, including amygdalin, cordyceps polysaccharide and gypenoside, respecitively. Liver fibrosis and function were assesed by histopathological examination, Western blot and serum biochemical detection. Metabolic profiling of liver tissue, serum and urine in each group were detected by gas chromatography-mass spectrometry (GC-MS) and transcriptomic changes were tested by gene chip. RT-qPCR was used to validate levels of different expressed genes (DEGs) with statistical significance. Metabolic network together with DEGs was constructed based on KEGG database.

Results: FZHY effectively improved liver fibrosis better than the mixture or single use of gypenoside, cordyceps sinensis mycelia and amygdalin. FZHY treatment widely modulated the metabolic profiles perturbed by liver fibrosis, involving several important metabolic pathways, including glycolysis/gluconeogenesis, glucose-alanine cycle, citrate cycle, galactose metabolism, tryptophan metabolism, urea cycle, etc. It also increased alanine and decreased glucose levels in liver tissue and decreased both of them in serum and urine, which were dysregulated by CCl treatment. Additionally, FZHY also upregulated expression of metabolic enzymes including Hk2, Adh1 and Gpt increased, and downregulated Gs and Acss2.

Conclusion: FZHY improved liver fibrosis in rats via altering the metabolic pathways and regulating gene expression of involved metabolic enzymes.

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Source
https://linkinghub.elsevier.com/retrieve/pii/S03788741183400
Publisher Site
http://dx.doi.org/10.1016/j.jep.2019.111888DOI Listing
June 2019
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