Interaction of RNA viruses of the natural virome with the African malaria vector, Anopheles coluzzii.

Authors:
Ferdinand Nanfack-Minkeu
Ferdinand Nanfack-Minkeu
Department of Parasites and Insect Vectors
Christian Mitri
Christian Mitri
University of Montpellier I and II
France
Eugeni Belda
Eugeni Belda
Universitat de València
Spain
Kenneth D Vernick
Kenneth D Vernick
University of Minnesota
United States

Sci Rep 2019 Apr 19;9(1):6319. Epub 2019 Apr 19.

Department of Parasites and Insect Vectors, Unit of Genetics and Genomics of Insect Vectors, Institut Pasteur, Paris, 75724 CEDEX 15, France.

Mosquitoes are colonized by a little-studied natural virome. Like the bacterial microbiome, the virome also probably influences the biology and immunity of mosquito vector populations, but tractable experimental models are lacking. We recently discovered two novel viruses in the virome of wild Anopheles and in colonies of the malaria vector Anopheles coluzzii: Anopheles C virus and Anopheles cypovirus. Here, we describe biological interactions between these two viruses and An. coluzzii mosquitoes. Viral abundance varies reproducibly during mosquito development. DNA forms of these viruses were not detected, and thus viral persistence is likely based on vertical transmission of RNA genomes. At least Anopheles C virus is vertically transmitted by an intraembryonic route. Relative abundance of the two viruses is inversely correlated in individual mosquitoes. One possible mechanism for this could be interactions with host immunity, and functional genomic analysis indicated differential influence of at least the Toll and JAK/STAT immune signaling pathways upon the viruses. The nonrandom distributions and interactions with host immunity suggest that these and other members of the natural virome may constitute a source of unrecognized heterogeneity in mosquito vector populations.

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-019-42825-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6474895PMC
April 2019

Publication Analysis

Top Keywords

natural virome
12
vector anopheles
8
malaria vector
8
anopheles coluzzii
8
vector populations
8
mosquito vector
8
interactions host
8
host immunity
8
anopheles virus
8
coluzzii mosquitoes
8
viruses
6
anopheles
6
virome
5
interactions viruses
4
transmission rna
4
virus vertically
4
rna genomes
4
vertically transmitted
4
genomes anopheles
4
intraembryonic route
4

References

(Supplied by CrossRef)
Article in J Vector Borne Dis
EJ Muturi et al.
J Vector Borne Dis 2006
Article in Mol Ecol Resour
J Villinger et al.
Mol Ecol Resour 2017
Article in Virology
JR Fauver et al.
Virology 2016
Article in Viruses
Ferdinand Nanfack Minkeu et al.
Viruses 2018
Article in Trans R Soc Trop Med Hyg
MC Williams et al.
Trans R Soc Trop Med Hyg 1965
Article in J Infect Dis
EJ Sanders et al.
J Infect Dis 1999
Article in Emerg Infect Dis
EB Rwaguma et al.
Emerg Infect Dis 1997
Article in PLoS Negl Trop Dis
CL Su et al.
PLoS Negl Trop Dis 2014
Article in Vet Ital
G Mancini et al.
Vet Ital 2017
Article in Proc Natl Acad Sci USA
KM Keene et al.
Proc Natl Acad Sci USA 2004

Similar Publications