Fungi participate in the dysbiosis of gut microbiota in patients with primary sclerosing cholangitis.

Authors:
Sara Lemoinne
Sara Lemoinne
Sorbonne Universités
France
Astrid Kemgang
Astrid Kemgang
Université Pierre et Marie Curie Paris 6
France
Marjolene Straube
Marjolene Straube
Sorbonne Universités
Christophe Corpechot
Christophe Corpechot
Service d'Hépatologie
Olivier Chazouilleres
Olivier Chazouilleres
Saint-Antoine University Hospital
France

Gut 2019 Apr 19. Epub 2019 Apr 19.

Centre de Recherche Saint-Antoine (CRSA), Sorbonne Université, INSERM, Paris, France.

Objective: Patients with primary sclerosing cholangitis (PSC) were previously shown to display a bacterial gut dysbiosis but fungal microbiota has never been examined in these patients. The aim of this study was to assess the fungal gut microbiota in patients with PSC.

Design: We analysed the faecal microbiota of patients with PSC and concomitant IBD (n=27), patients with PSC and no IBD (n=22), patients with IBD and no PSC (n=33) and healthy subjects (n=30). Bacterial and fungal composition of the faecal microbiota was determined using 16S and ITS2 sequencing, respectively.

Results: We found that patients with PSC harboured bacterial dysbiosis characterised by a decreased biodiversity, an altered composition and a decreased correlation network density. These alterations of the microbiota were associated with PSC, independently of IBD status. For the first time, we showed that patients with PSC displayed a fungal gut dysbiosis, characterised by a relative increase in biodiversity and an altered composition. Notably, we observed an increased proportion of and a decreased proportion of . Compared with patients with IBD and healthy subjects, the gut microbiota of patients with PSC exhibited a strong disruption in bacteria-fungi correlation network, suggesting an alteration in the interkingdom crosstalk.

Conclusion: This study demonstrates that bacteria and fungi contribute to gut dysbiosis in PSC.

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Source
http://dx.doi.org/10.1136/gutjnl-2018-317791DOI Listing

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April 2019
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