Co-Delivery of M2e Virus-Like Particles with Influenza Split Vaccine to the Skin Using Microneedles Enhances the Efficacy of Cross Protection.

Authors:
Min-Chul Kim
Min-Chul Kim
Georgia State University
United States
Ki-Hye Kim
Ki-Hye Kim
Chungnam National University
South Korea
Jeong Woo Lee
Jeong Woo Lee
Georgia Institute of Technology
United States
Yu-Na Lee
Yu-Na Lee
Konkuk University
Hyo-Jick Choi
Hyo-Jick Choi
University of Alberta
Canada
Yu-jin Kim
Yu-jin Kim
Seoul National University Bundang Hospital
Richard W Compans
Richard W Compans
Emory University School of Medicine
United States

Pharmaceutics 2019 Apr 18;11(4). Epub 2019 Apr 18.

Institute for Biomedical Sciences, Georgia State University, Atlanta, GA 30303, USA.

It is a high priority to develop a simple and effective delivery method for a cross-protective influenza vaccine. We investigated skin immunization by microneedle (MN) patch with human influenza split vaccine and virus-like particles containing heterologous M2 extracellular (M2e) domains (M2e5x virus-like particles (VLP)) as a cross-protective influenza vaccine candidate. Co-delivery of influenza split vaccine and M2e5x VLP to the skin by MN patch was found to confer effective protection against heterosubtypic influenza virus by preventing weight loss and reducing lung viral loads. Compared to intramuscular immunization, MN-based delivery of combined split vaccine and M2e5x VLPs shaped cellular immune responses toward T helper type 1 responses increasing IgG2a isotype antibodies as well as IFN-γ producing cells in mucosal and systemic sites. This study provides evidence that potential immunological and logistic benefits of M2e5x VLP with human influenza split vaccine delivered by MN patch can be used to develop an easy-to-administer cross-protective influenza vaccine.

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Source
http://dx.doi.org/10.3390/pharmaceutics11040188DOI Listing
April 2019

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