Chicken viperin inhibits Newcastle disease virus infection in vitro: A possible interaction with the viral matrix protein.

Authors:
Manisha Shah
Manisha Shah
Ohio State University
Canada
Anjali Gupta
Anjali Gupta
Sri Aurobindo College of Dentistry
Indore | India
Dr. Rakesh Kumar, PhD
Dr. Rakesh Kumar, PhD
Panjab University, chandigarh
India
Sachin Kumar
Sachin Kumar
Indian Institute of Technology Guwahati
Guwahati | India

Cytokine 2019 Apr 16;120:28-40. Epub 2019 Apr 16.

Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India. Electronic address:

Viperin is an interferon-inducible protein that helps in protecting mammals against various virus infections. Viperin is a highly conserved member of the interferon-stimulated genes (ISG) family in many species. Viperin has been shown to play a pivotal role in the innate immunity of chicken; however, its role has not been explored in its antiviral potential. Newcastle disease virus (NDV) is the causative agent of an infectious disease in poultry. In the present study, we have shown the anti-NDV effect of chicken viperin (cViperin). The impact of cViperin upon NDV infection was investigated in chicken embryo fibroblast. The modeling of the cViperin protein was done using I-TASSER and ZDOCK is used to predict the possible interaction with the matrix protein of NDV. The interaction was further confirmed by co-immunoprecipitation assay using recombinant matrix protein of NDV with the recombinant cViperin. The recombinant NDV expressing cViperin showed reduced replication of the virus upon its growth kinetics. Our results suggest downregulation of NDV replication in the presence of cViperin. The study will be critical to elaborate our understanding of the chicken innate immune system which could help develop antiviral strategies against NDV infection.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cyto.2019.04.007DOI Listing
April 2019
1 Read
2.664 Impact Factor

Publication Analysis

Top Keywords

matrix protein
12
protein ndv
8
newcastle disease
8
ndv infection
8
disease virus
8
chicken viperin
8
ndv
7
cviperin
6
protein
5
chicken
5
ndv interaction
4
fibroblast modeling
4
interaction confirmed
4
confirmed co-immunoprecipitation
4
embryo fibroblast
4
modeling cviperin
4
cviperin protein
4
zdock predict
4
chicken embryo
4
i-tasser zdock
4

Similar Publications