Age-dependent variations in the expression of myosin isoforms and myogenic factors during the involution of the proximal sesamoidean ligament of sheep.

Authors:
Lina Cancellara
Lina Cancellara
University of Padova
Luana Toniolo
Luana Toniolo
University of Padova
Carlo Reggiani
Carlo Reggiani
University of Padova
Italy
Francesco Mascarello
Francesco Mascarello
University of Padova
Italy
Luca Melotti
Luca Melotti
University of Bristol
United Kingdom
Maura Francolini
Maura Francolini
Università degli Studi di Milano
Milano | Italy
Lisa Maccatrozzo
Lisa Maccatrozzo
Interuniversity Institute of Myology (IIM)

Res Vet Sci 2019 Apr 11;124:270-279. Epub 2019 Apr 11.

Department of Comparative Biomedicine and Food Science, Università di Padova, Italy. Electronic address:

In ungulates the stability of the fetlock joint is dependent on several muscles, which are exposed to high stress and strain. Among those muscles, the proximal sesamoidean ligament or PSL (also known as the suspensory ligament or Ruini's elasto-tendinous organ) is organized at birth in layers of muscle fibres alternated with abundant tendinous tissue that, during the postnatal development, becomes the predominant tissue. In this study we analysed the PSL of the sheep at the age of 1, 30 and 180 days and determined the expression of several genes which either (a) are markers of muscle fibre growth and maturation, or (b) play a role as signal molecules. We observed an accelerated maturation, as indicated by the transition of MyHC isoform expression towards the slow isoforms and a reduced regenerative potential indicated by the low Pax7 expression and the altered Wnt signalling. We also found a specific myogenic expression pattern of MyoD, Myf5 and Myogenin in the developing PSL and high mRNA levels of specific fibrogenic factors, as TGF-β1, that, undoubtedly, stimulate the growth of connective tissue. Our observations confirmed, at molecular level, the peculiarity of the fast involution observed in PSL a muscle that undergoes a very specific active differentiation process during early development, which implies myofibres involution and their replacement with connective tissue.

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http://dx.doi.org/10.1016/j.rvsc.2019.04.006DOI Listing
April 2019
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